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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Wolbachia sp. subsp. Drosophila simulans Porphobilinogen deaminase (hemC) CSB-YP010524WBP
CSB-EP010524WBP
CSB-BP010524WBP
CSB-MP010524WBP
CSB-EP010524WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans ATP synthase subunit delta (atpH) CSB-YP002355WBP
CSB-EP002355WBP
CSB-BP002355WBP
CSB-MP002355WBP
CSB-EP002355WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Methionyl-tRNA formyltransferase (fmt) CSB-YP015150WBP
CSB-EP015150WBP
CSB-BP015150WBP
CSB-MP015150WBP
CSB-EP015150WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Ribonuclease 3 (rnc) CSB-YP019809WBP
CSB-EP019809WBP
CSB-BP019809WBP
CSB-MP019809WBP
CSB-EP019809WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Thymidylate synthase (thyA) CSB-YP025393BWT
CSB-EP025393BWT
CSB-BP025393BWT
CSB-MP025393BWT
CSB-EP025393BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203BWT
CSB-EP001203BWT
CSB-BP001203BWT
CSB-MP001203BWT
CSB-EP001203BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Peptide chain release factor 1 (prfA) CSB-YP015197BWT
CSB-EP015197BWT
CSB-BP015197BWT
CSB-MP015197BWT
CSB-EP015197BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Adenosylhomocysteinase (ahcY) CSB-YP001474BWT
CSB-EP001474BWT
CSB-BP001474BWT
CSB-MP001474BWT
CSB-EP001474BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451BWT
CSB-EP015451BWT
CSB-BP015451BWT
CSB-MP015451BWT
CSB-EP015451BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824BWT
CSB-EP005824BWT
CSB-BP005824BWT
CSB-MP005824BWT
CSB-EP005824BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Methionyl-tRNA formyltransferase (fmt) CSB-YP015150BWT
CSB-EP015150BWT
CSB-BP015150BWT
CSB-MP015150BWT
CSB-EP015150BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brevibacillus brevis S-adenosylmethionine decarboxylase proenzyme (speD), partial CSB-YP001658BWL
CSB-EP001658BWL
CSB-BP001658BWL
CSB-MP001658BWL
CSB-EP001658BWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brevibacillus brevis ATP synthase subunit beta (atpD) CSB-YP002350BWL
CSB-EP002350BWL
CSB-BP002350BWL
CSB-MP002350BWL
CSB-EP002350BWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brevibacillus brevis Ribosome-recycling factor (frr) CSB-YP014941BWL
CSB-EP014941BWL
CSB-BP014941BWL
CSB-MP014941BWL
CSB-EP014941BWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Elongation factor Tu (tuf) CSB-YP025342RLK
CSB-EP025342RLK
CSB-BP025342RLK
CSB-MP025342RLK
CSB-EP025342RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis ATP synthase subunit alpha (atpA), partial CSB-YP002344RLK
CSB-EP002344RLK
CSB-BP002344RLK
CSB-MP002344RLK
CSB-EP002344RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gemmatimonas aurantiaca Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917GFG
CSB-EP022917GFG
CSB-BP022917GFG
CSB-MP022917GFG
CSB-EP022917GFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Nucleoside diphosphate kinase (ndk) CSB-YP015889MVI
CSB-EP015889MVI
CSB-BP015889MVI
CSB-MP015889MVI
CSB-EP015889MVI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Thymidylate synthase (thyA) CSB-YP025393MVI
CSB-EP025393MVI
CSB-BP025393MVI
CSB-MP025393MVI
CSB-EP025393MVI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824MVI
CSB-EP005824MVI
CSB-BP005824MVI
CSB-MP005824MVI
CSB-EP005824MVI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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