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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter sp. Elongation factor Ts (tsf) CSB-YP025124GFP
CSB-EP025124GFP
CSB-BP025124GFP
CSB-MP025124GFP
CSB-EP025124GFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax ebreus Acyl carrier protein (acpP) CSB-YP015636AWK
CSB-EP015636AWK
CSB-BP015636AWK
CSB-MP015636AWK
CSB-EP015636AWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax ebreus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150AWK
CSB-EP015150AWK
CSB-BP015150AWK
CSB-MP015150AWK
CSB-EP015150AWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaerocellum thermophilum tRNA dimethylallyltransferase (miaA) CSB-YP024529BYP
CSB-EP024529BYP
CSB-BP024529BYP
CSB-MP024529BYP
CSB-EP024529BYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaerocellum thermophilum Ribosome-recycling factor (frr) CSB-YP014941BYP
CSB-EP014941BYP
CSB-BP014941BYP
CSB-MP014941BYP
CSB-EP014941BYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaerocellum thermophilum Peptide chain release factor 1 (prfA) CSB-YP015197BYP
CSB-EP015197BYP
CSB-BP015197BYP
CSB-MP015197BYP
CSB-EP015197BYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaerocellum thermophilum Argininosuccinate synthase (argG) CSB-YP002234BYP
CSB-EP002234BYP
CSB-BP002234BYP
CSB-MP002234BYP
CSB-EP002234BYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Ubiquitin-like modifier-activating enzyme 5 CSB-YP025415BTT
CSB-EP025415BTT
CSB-BP025415BTT
CSB-MP025415BTT
CSB-EP025415BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida dubliniensis Methylthioribose-1-phosphate isomerase (MRI1) CSB-YP014799CZH
CSB-EP014799CZH
CSB-BP014799CZH
CSB-MP014799CZH
CSB-EP014799CZH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar F-box/LRR-repeat protein 5 (fbxl5), partial CSB-YP008477SWI
CSB-EP008477SWI
CSB-BP008477SWI
CSB-MP008477SWI
CSB-EP008477SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. equi Thymidylate kinase (tmk) CSB-YP007226FMI
CSB-EP007226FMI
CSB-BP007226FMI
CSB-MP007226FMI
CSB-EP007226FMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. equi tRNA pseudouridine synthase A (truA) CSB-YP019086FMI
CSB-EP019086FMI
CSB-BP019086FMI
CSB-MP019086FMI
CSB-EP019086FMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. equi Translation initiation factor IF-2 (infB), partial CSB-YP015164FMI
CSB-EP015164FMI
CSB-BP015164FMI
CSB-MP015164FMI
CSB-EP015164FMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrococcus caseolyticus Serine hydroxymethyltransferase (glyA) CSB-YP021274MPA
CSB-EP021274MPA
CSB-BP021274MPA
CSB-MP021274MPA
CSB-EP021274MPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrococcus caseolyticus Peptide deformylase (def) CSB-YP017707MPA
CSB-EP017707MPA
CSB-BP017707MPA
CSB-MP017707MPA
CSB-EP017707MPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrococcus caseolyticus Elongation factor Ts (tsf) CSB-YP025124MPA
CSB-EP025124MPA
CSB-BP025124MPA
CSB-MP025124MPA
CSB-EP025124MPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse CAP-Gly domain-containing linker protein 3 (Clip3) CSB-YP005555MO
CSB-EP005555MO
CSB-BP005555MO
CSB-MP005555MO
CSB-EP005555MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Ubiquitin-fold modifier-conjugating enzyme 1 (ufc1) CSB-YP025558SWI
CSB-EP025558SWI
CSB-BP025558SWI
CSB-MP025558SWI
CSB-EP025558SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit beta (atpD) CSB-YP002350BQQ
CSB-EP002350BQQ
CSB-BP002350BQQ
CSB-MP002350BQQ
CSB-EP002350BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917BQQ
CSB-EP022917BQQ
CSB-BP022917BQQ
CSB-MP022917BQQ
CSB-EP022917BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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