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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chloroflexus aurantiacus Ribosome-recycling factor (frr) CSB-YP014941DSW
CSB-EP014941DSW
CSB-BP014941DSW
CSB-MP014941DSW
CSB-EP014941DSW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Galactokinase (galK) CSB-YP009198DSW
CSB-EP009198DSW
CSB-BP009198DSW
CSB-MP009198DSW
CSB-EP009198DSW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrococcus caseolyticus Peptide chain release factor 1 (prfA) CSB-YP015197MPA
CSB-EP015197MPA
CSB-BP015197MPA
CSB-MP015197MPA
CSB-EP015197MPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrococcus caseolyticus Lipoyl synthase (lipA) CSB-YP012927MPA
CSB-EP012927MPA
CSB-BP012927MPA
CSB-MP012927MPA
CSB-EP012927MPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar SOSS complex subunit C (ssbip1) CSB-YP004350SWI
CSB-EP004350SWI
CSB-BP004350SWI
CSB-MP004350SWI
CSB-EP004350SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Serine hydroxymethyltransferase (glyA) CSB-YP021274BQQ
CSB-EP021274BQQ
CSB-BP021274BQQ
CSB-MP021274BQQ
CSB-EP021274BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ornithine aminotransferase (rocD) CSB-YP016242BQQ
CSB-EP016242BQQ
CSB-BP016242BQQ
CSB-MP016242BQQ
CSB-EP016242BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Urocanate hydratase (hutU), partial CSB-YP025677BQQ
CSB-EP025677BQQ
CSB-BP025677BQQ
CSB-MP025677BQQ
CSB-EP025677BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Translation initiation factor IF-2 (infB), partial CSB-YP015164BQQ
CSB-EP015164BQQ
CSB-BP015164BQQ
CSB-MP015164BQQ
CSB-EP015164BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease 3 (rnc) CSB-YP019809BQQ
CSB-EP019809BQQ
CSB-BP019809BQQ
CSB-MP019809BQQ
CSB-EP019809BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lipoyl synthase (lipA) CSB-YP012927BQQ
CSB-EP012927BQQ
CSB-BP012927BQQ
CSB-MP012927BQQ
CSB-EP012927BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium radiobacter Thymidylate synthase (thyA) CSB-YP025393AYR
CSB-EP025393AYR
CSB-BP025393AYR
CSB-MP025393AYR
CSB-EP025393AYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis tRNA pseudouridine synthase A (truA) CSB-YP019086AYU
CSB-EP019086AYU
CSB-BP019086AYU
CSB-MP019086AYU
CSB-EP019086AYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis Ferrochelatase (hemH) CSB-YP008579AYU
CSB-EP008579AYU
CSB-BP008579AYU
CSB-MP008579AYU
CSB-EP008579AYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis Peptide chain release factor 1 (prfA) CSB-YP015197AYU
CSB-EP015197AYU
CSB-BP015197AYU
CSB-MP015197AYU
CSB-EP015197AYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis Ribonuclease 3 (rnc) CSB-YP019809AYU
CSB-EP019809AYU
CSB-BP019809AYU
CSB-MP019809AYU
CSB-EP019809AYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis Glycine cleavage system H protein (gcvH) CSB-YP009335AYU
CSB-EP009335AYU
CSB-BP009335AYU
CSB-MP009335AYU
CSB-EP009335AYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis Thymidylate synthase (thyA) CSB-YP025393AYU
CSB-EP025393AYU
CSB-BP025393AYU
CSB-MP025393AYU
CSB-EP025393AYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga neapolitana ATP synthase subunit delta (atpH) CSB-YP002355TNL
CSB-EP002355TNL
CSB-BP002355TNL
CSB-MP002355TNL
CSB-EP002355TNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga neapolitana Ribosome-recycling factor (frr) CSB-YP014941TNL
CSB-EP014941TNL
CSB-BP014941TNL
CSB-MP014941TNL
CSB-EP014941TNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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