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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmo salar E3 ubiquitin-protein ligase rnf146 (rnf146) CSB-YP019843SWI
CSB-EP019843SWI
CSB-BP019843SWI
CSB-MP019843SWI
CSB-EP019843SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. equi ATP synthase subunit a (atpB), partial CSB-YP015070FMI1
CSB-EP015070FMI1
CSB-BP015070FMI1
CSB-MP015070FMI1
CSB-EP015070FMI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. equi ATP synthase subunit beta (atpD) CSB-YP002350FMI
CSB-EP002350FMI
CSB-BP002350FMI
CSB-MP002350FMI
CSB-EP002350FMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. equi Elongation factor G (fusA), partial CSB-YP009373FMI
CSB-EP009373FMI
CSB-BP009373FMI
CSB-MP009373FMI
CSB-EP009373FMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. equi Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146FMI
CSB-EP019146FMI
CSB-BP019146FMI
CSB-MP019146FMI
CSB-EP019146FMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. equi GTPase Era (era) CSB-YP007759FMI
CSB-EP007759FMI
CSB-BP007759FMI
CSB-MP007759FMI
CSB-EP007759FMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrococcus caseolyticus Thymidine kinase (tdk) CSB-YP023577MPA
CSB-EP023577MPA
CSB-BP023577MPA
CSB-MP023577MPA
CSB-EP023577MPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrococcus caseolyticus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917MPA
CSB-EP022917MPA
CSB-BP022917MPA
CSB-MP022917MPA
CSB-EP022917MPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Flap endonuclease 1 (fen1) CSB-YP008585SWI
CSB-EP008585SWI
CSB-BP008585SWI
CSB-MP008585SWI
CSB-EP008585SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Acetyl-CoA carboxylase 2 (ACC2), partial CSB-YP001120OFG
CSB-EP001120OFG
CSB-BP001120OFG
CSB-MP001120OFG
CSB-EP001120OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit alpha (atpA), partial CSB-YP002344BQQ
CSB-EP002344BQQ
CSB-BP002344BQQ
CSB-MP002344BQQ
CSB-EP002344BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thymidine kinase (tdk) CSB-YP023577BQQ
CSB-EP023577BQQ
CSB-BP023577BQQ
CSB-MP023577BQQ
CSB-EP023577BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA dimethylallyltransferase (miaA) CSB-YP024529BQQ
CSB-EP024529BQQ
CSB-BP024529BQQ
CSB-MP024529BQQ
CSB-EP024529BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824BQQ
CSB-EP005824BQQ
CSB-BP005824BQQ
CSB-MP005824BQQ
CSB-EP005824BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycine cleavage system H protein (gcvH) CSB-YP009335BQQ
CSB-EP009335BQQ
CSB-BP009335BQQ
CSB-MP009335BQQ
CSB-EP009335BQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium radiobacter Transaldolase (tal) CSB-YP023112AYR
CSB-EP023112AYR
CSB-BP023112AYR
CSB-MP023112AYR
CSB-EP023112AYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis Serine hydroxymethyltransferase (glyA) CSB-YP021274AYU
CSB-EP021274AYU
CSB-BP021274AYU
CSB-MP021274AYU
CSB-EP021274AYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis tRNA dimethylallyltransferase (miaA) CSB-YP024529AYU
CSB-EP024529AYU
CSB-BP024529AYU
CSB-MP024529AYU
CSB-EP024529AYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga neapolitana ATP synthase subunit beta (atpD) CSB-YP002350TNL
CSB-EP002350TNL
CSB-BP002350TNL
CSB-MP002350TNL
CSB-EP002350TNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter lari Nucleoside diphosphate kinase (ndk) CSB-YP015889CZB
CSB-EP015889CZB
CSB-BP015889CZB
CSB-MP015889CZB
CSB-EP015889CZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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