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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clavibacter michiganensis subsp. sepedonicus Lipoyl synthase (lipA) CSB-YP012927DTV
CSB-EP012927DTV
CSB-BP012927DTV
CSB-MP012927DTV
CSB-EP012927DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Serine hydroxymethyltransferase (glyA) CSB-YP021274DTV
CSB-EP021274DTV
CSB-BP021274DTV
CSB-MP021274DTV
CSB-EP021274DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum Elongation factor 2 (fusA), partial CSB-YP007434HTL
CSB-EP007434HTL
CSB-BP007434HTL
CSB-MP007434HTL
CSB-EP007434HTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum Flap endonuclease 1 (fen) CSB-YP008585HTL
CSB-EP008585HTL
CSB-BP008585HTL
CSB-MP008585HTL
CSB-EP008585HTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida GTPase Era (era) CSB-YP007759FGA
CSB-EP007759FGA
CSB-BP007759FGA
CSB-MP007759FGA
CSB-EP007759FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callithrix jacchus Cadherin-2 (CDH2), partial CSB-YP005045CYL1
CSB-EP005045CYL1
CSB-BP005045CYL1
CSB-MP005045CYL1
CSB-EP005045CYL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033FGA
CSB-EP018033FGA
CSB-BP018033FGA
CSB-MP018033FGA
CSB-EP018033FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Porphobilinogen deaminase (hemC) CSB-YP010524FGA
CSB-EP010524FGA
CSB-BP010524FGA
CSB-MP010524FGA
CSB-EP010524FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus tRNA pseudouridine synthase A (truA) CSB-YP019086TMI
CSB-EP019086TMI
CSB-BP019086TMI
CSB-MP019086TMI
CSB-EP019086TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus Acyl carrier protein (acpP) CSB-YP015636TMI
CSB-EP015636TMI
CSB-BP015636TMI
CSB-MP015636TMI
CSB-EP015636TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus Elongation factor Ts (tsf) CSB-YP025124TMI
CSB-EP025124TMI
CSB-BP025124TMI
CSB-MP025124TMI
CSB-EP025124TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus Glucose-6-phosphate isomerase (pgi) CSB-YP009717TMI
CSB-EP009717TMI
CSB-BP009717TMI
CSB-MP009717TMI
CSB-EP009717TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter sp. GTPase Era (era) CSB-YP007759TMJ
CSB-EP007759TMJ
CSB-BP007759TMJ
CSB-MP007759TMJ
CSB-EP007759TMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter sp. 6-phosphofructokinase (pfkA) CSB-YP017822TMJ
CSB-EP017822TMJ
CSB-BP017822TMJ
CSB-MP017822TMJ
CSB-EP017822TMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter sp. Elongation factor G (fusA), partial CSB-YP009373TMJ
CSB-EP009373TMJ
CSB-BP009373TMJ
CSB-MP009373TMJ
CSB-EP009373TMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein arginine N-methyltransferase 6 (prmt6) CSB-YP018735XBF
CSB-EP018735XBF
CSB-BP018735XBF
CSB-MP018735XBF
CSB-EP018735XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein spinster homolog 2 (spns2), partial CSB-YP022592XBF
CSB-EP022592XBF
CSB-BP022592XBF
CSB-MP022592XBF
CSB-EP022592XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcystis aeruginosa tRNA dimethylallyltransferase (miaA) CSB-YP024529MTQ
CSB-EP024529MTQ
CSB-BP024529MTQ
CSB-MP024529MTQ
CSB-EP024529MTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Sex comb on midleg-like protein 1 (SCML1) CSB-YP020829GGZ
CSB-EP020829GGZ
CSB-BP020829GGZ
CSB-MP020829GGZ
CSB-EP020829GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aedes aegypti NADH-ubiquinone oxidoreductase chain 5 (mt:ND5), partial CSB-YP015081AXQ
CSB-EP015081AXQ
CSB-BP015081AXQ
CSB-MP015081AXQ
CSB-EP015081AXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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