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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Putative RNA polymerase II subunit B1 CTD phosphatase rpap2 (rpap2), partial CSB-YP020095DIL
CSB-EP020095DIL
CSB-BP020095DIL
CSB-MP020095DIL
CSB-EP020095DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus somnus Peptide deformylase (def) CSB-YP017707HTE
CSB-EP017707HTE
CSB-BP017707HTE
CSB-MP017707HTE
CSB-EP017707HTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus somnus ATP synthase subunit b (atpF), partial CSB-YP002358HTE1
CSB-EP002358HTE1
CSB-BP002358HTE1
CSB-MP002358HTE1
CSB-EP002358HTE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus somnus Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033HTE
CSB-EP018033HTE
CSB-BP018033HTE
CSB-MP018033HTE
CSB-EP018033HTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus somnus Elongation factor G (fusA), partial CSB-YP009373HTE
CSB-EP009373HTE
CSB-BP009373HTE
CSB-MP009373HTE
CSB-EP009373HTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus somnus Argininosuccinate synthase (argG) CSB-YP002234HTE
CSB-EP002234HTE
CSB-BP002234HTE
CSB-MP002234HTE
CSB-EP002234HTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium sp. Nucleoside diphosphate kinase (ndk) CSB-YP015889MTE
CSB-EP015889MTE
CSB-BP015889MTE
CSB-MP015889MTE
CSB-EP015889MTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium sp. ATP synthase subunit alpha (atpA), partial CSB-YP002344MTE
CSB-EP002344MTE
CSB-BP002344MTE
CSB-MP002344MTE
CSB-EP002344MTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium sp. Elongation factor Ts (tsf) CSB-YP025124MTE
CSB-EP025124MTE
CSB-BP025124MTE
CSB-MP025124MTE
CSB-EP025124MTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium sp. Peptide chain release factor 1 (prfA) CSB-YP015197MTE
CSB-EP015197MTE
CSB-BP015197MTE
CSB-MP015197MTE
CSB-EP015197MTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033XBM
CSB-EP018033XBM
CSB-BP018033XBM
CSB-MP018033XBM
CSB-EP018033XBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP synthase subunit alpha (atpA), partial CSB-YP002344XBM
CSB-EP002344XBM
CSB-BP002344XBM
CSB-MP002344XBM
CSB-EP002344XBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Methionyl-tRNA formyltransferase (fmt) CSB-YP015150XBM
CSB-EP015150XBM
CSB-BP015150XBM
CSB-MP015150XBM
CSB-EP015150XBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Enolase-phosphatase E1 (mtnC) CSB-YP007674XBM
CSB-EP007674XBM
CSB-BP007674XBM
CSB-MP007674XBM
CSB-EP007674XBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella philomiragia subsp. philomiragia Elongation factor G (fusA), partial CSB-YP009373FFC
CSB-EP009373FFC
CSB-BP009373FFC
CSB-MP009373FFC
CSB-EP009373FFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella philomiragia subsp. philomiragia Nucleoside diphosphate kinase (ndk) CSB-YP015889FFC
CSB-EP015889FFC
CSB-BP015889FFC
CSB-MP015889FFC
CSB-EP015889FFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella philomiragia subsp. philomiragia ATP synthase subunit delta (atpH) CSB-YP002355FFC
CSB-EP002355FFC
CSB-BP002355FFC
CSB-MP002355FFC
CSB-EP002355FFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella halifaxensis Phosphoserine aminotransferase (serC) CSB-YP018838SYU
CSB-EP018838SYU
CSB-BP018838SYU
CSB-MP018838SYU
CSB-EP018838SYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella halifaxensis Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493SYU
CSB-EP009493SYU
CSB-BP009493SYU
CSB-MP009493SYU
CSB-EP009493SYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella halifaxensis Elongation factor Ts (tsf) CSB-YP025124SYU
CSB-EP025124SYU
CSB-BP025124SYU
CSB-MP025124SYU
CSB-EP025124SYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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