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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anti-sigma F factor antagonist (spoIIAA) CSB-YP300921BQR
CSB-EP300921BQR
CSB-BP300921BQR
CSB-MP300921BQR
CSB-EP300921BQR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variable large protein 9 (vlp9) CSB-YP300922BIP
CSB-EP300922BIP
CSB-BP300922BIP
CSB-MP300922BIP
CSB-EP300922BIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variable large protein 18 (vlp18) CSB-YP300923BIP
CSB-EP300923BIP
CSB-BP300923BIP
CSB-MP300923BIP
CSB-EP300923BIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus megaterium Indole-3-glycerol phosphate synthase (trpC) CSB-YP300924BQW
CSB-EP300924BQW
CSB-BP300924BQW
CSB-MP300924BQW
CSB-EP300924BQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yitZ (yitZ), partial CSB-YP300925BRJ1
CSB-EP300925BRJ1
CSB-BP300925BRJ1
CSB-MP300925BRJ1
CSB-EP300925BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ywmF (ywmF), partial CSB-YP300926BRJ1
CSB-EP300926BRJ1
CSB-BP300926BRJ1
CSB-MP300926BRJ1
CSB-EP300926BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable tautomerase ywhB (ywhB) CSB-YP300928BRJ
CSB-EP300928BRJ
CSB-BP300928BRJ
CSB-MP300928BRJ
CSB-EP300928BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywhH (ywhH) CSB-YP300929BRJ
CSB-EP300929BRJ
CSB-BP300929BRJ
CSB-MP300929BRJ
CSB-EP300929BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywhK (ywhK) CSB-YP300930BRJ
CSB-EP300930BRJ
CSB-BP300930BRJ
CSB-MP300930BRJ
CSB-EP300930BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Antilisterial bacteriocin subtilosin biosynthesis protein AlbB (albB), partial CSB-YP300931BRJ
CSB-EP300931BRJ
CSB-BP300931BRJ
CSB-MP300931BRJ
CSB-EP300931BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphate acyltransferase (plsX) CSB-YP300932BRJ
CSB-EP300932BRJ
CSB-BP300932BRJ
CSB-MP300932BRJ
CSB-EP300932BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative HTH-type transcriptional regulator ywnA (ywnA) CSB-YP300933BRJ
CSB-EP300933BRJ
CSB-BP300933BRJ
CSB-MP300933BRJ
CSB-EP300933BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywnG (ywnG), partial CSB-YP300934BRJ1
CSB-EP300934BRJ1
CSB-BP300934BRJ1
CSB-MP300934BRJ1
CSB-EP300934BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YveK (yveK), partial CSB-YP300935BRJ1
CSB-EP300935BRJ1
CSB-BP300935BRJ1
CSB-MP300935BRJ1
CSB-EP300935BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transmembrane protein epsG (epsG), partial CSB-YP300936BRJ
CSB-EP300936BRJ
CSB-BP300936BRJ
CSB-MP300936BRJ
CSB-EP300936BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative pyruvyl transferase epsO (epsO) CSB-YP300937BRJ
CSB-EP300937BRJ
CSB-BP300937BRJ
CSB-MP300937BRJ
CSB-EP300937BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ygaB (ygaB) CSB-YP300938BRJ
CSB-EP300938BRJ
CSB-BP300938BRJ
CSB-MP300938BRJ
CSB-EP300938BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized peptidase ygaJ (ygaJ) CSB-YP300939BRJ
CSB-EP300939BRJ
CSB-BP300939BRJ
CSB-MP300939BRJ
CSB-EP300939BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Flavodoxin (DvMF_1143) CSB-YP300940DJR
CSB-EP300940DJR
CSB-BP300940DJR
CSB-MP300940DJR
CSB-EP300940DJR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative colanic biosynthesis UDP-glucose lipid carrier transferase (wcaJ), partial CSB-YP300941ENV1
CSB-EP300941ENV1
CSB-BP300941ENV1
CSB-MP300941ENV1
CSB-EP300941ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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