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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Putative uncharacterized protein YbcD (ybcD) CSB-YP300595ENV
CSB-EP300595ENV
CSB-BP300595ENV
CSB-MP300595ENV
CSB-EP300595ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ydaV (ydaV) CSB-YP300596ENV
CSB-EP300596ENV
CSB-BP300596ENV
CSB-MP300596ENV
CSB-EP300596ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein ydeP (ydeP) CSB-YP300597ENV
CSB-EP300597ENV
CSB-BP300597ENV
CSB-MP300597ENV
CSB-EP300597ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP300598ENV
CSB-EP300598ENV
CSB-BP300598ENV
CSB-MP300598ENV
CSB-EP300598ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized fimbrial-like protein ydeQ (ydeQ) CSB-YP300599ENV
CSB-EP300599ENV
CSB-BP300599ENV
CSB-MP300599ENV
CSB-EP300599ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yagL (yagL) CSB-YP300600ENV
CSB-EP300600ENV
CSB-BP300600ENV
CSB-MP300600ENV
CSB-EP300600ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ycjW (ycjW) CSB-YP300601ENV
CSB-EP300601ENV
CSB-BP300601ENV
CSB-MP300601ENV
CSB-EP300601ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybeR (ybeR) CSB-YP300602ENV
CSB-EP300602ENV
CSB-BP300602ENV
CSB-MP300602ENV
CSB-EP300602ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein SufA (sufA) CSB-YP300603ENV
CSB-EP300603ENV
CSB-BP300603ENV
CSB-MP300603ENV
CSB-EP300603ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli FeS cluster assembly protein sufD (sufD) CSB-YP300604ENV
CSB-EP300604ENV
CSB-BP300604ENV
CSB-MP300604ENV
CSB-EP300604ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ferredoxin-like protein ydiT (ydiT) CSB-YP300605ENV
CSB-EP300605ENV
CSB-BP300605ENV
CSB-MP300605ENV
CSB-EP300605ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ureidoglycolate hydrolase (allA) CSB-YP300606ENV
CSB-EP300606ENV
CSB-BP300606ENV
CSB-MP300606ENV
CSB-EP300606ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Propionate catabolism operon regulatory protein (prpR) CSB-YP300607ENV
CSB-EP300607ENV
CSB-BP300607ENV
CSB-MP300607ENV
CSB-EP300607ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Spheroplast protein Y (spy) CSB-YP300608ENV
CSB-EP300608ENV
CSB-BP300608ENV
CSB-MP300608ENV
CSB-EP300608ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipoprotein yfgL (yfgL) CSB-YP300609ENV
CSB-EP300609ENV
CSB-BP300609ENV
CSB-MP300609ENV
CSB-EP300609ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maltose O-acetyltransferase (maa) CSB-YP300610ENV
CSB-EP300610ENV
CSB-BP300610ENV
CSB-MP300610ENV
CSB-EP300610ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli CinA-like protein (yfaY) CSB-YP300611ENV
CSB-EP300611ENV
CSB-BP300611ENV
CSB-MP300611ENV
CSB-EP300611ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 60 kDa chaperonin 1 (groL1), partial CSB-YP300612BVW
CSB-EP300612BVW
CSB-BP300612BVW
CSB-MP300612BVW
CSB-EP300612BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Probable cation-transporting ATPase HI_0290 (HI_0290), partial CSB-YP300614HTA
CSB-EP300614HTA
CSB-BP300614HTA
CSB-MP300614HTA
CSB-EP300614HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP300617NFZ
CSB-EP300617NFZ
CSB-BP300617NFZ
CSB-MP300617NFZ
CSB-EP300617NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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