Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Cardiolipin synthase (cls), partial CSB-YP005985ENP1
CSB-EP005985ENP1
CSB-BP005985ENP1
CSB-MP005985ENP1
CSB-EP005985ENP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Methionyl-tRNA formyltransferase (fmt) CSB-YP015150ENP
CSB-EP015150ENP
CSB-BP015150ENP
CSB-MP015150ENP
CSB-EP015150ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA dimethylallyltransferase (miaA) CSB-YP024529DUJ
CSB-EP024529DUJ
CSB-BP024529DUJ
CSB-MP024529DUJ
CSB-EP024529DUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146DUJ
CSB-EP019146DUJ
CSB-BP019146DUJ
CSB-MP019146DUJ
CSB-EP019146DUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Serine hydroxymethyltransferase (glyA) CSB-YP021274DUJ
CSB-EP021274DUJ
CSB-BP021274DUJ
CSB-MP021274DUJ
CSB-EP021274DUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor Ts (tsf) CSB-YP025124DUJ
CSB-EP025124DUJ
CSB-BP025124DUJ
CSB-MP025124DUJ
CSB-EP025124DUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor G (fusA), partial CSB-YP009373DUJ
CSB-EP009373DUJ
CSB-BP009373DUJ
CSB-MP009373DUJ
CSB-EP009373DUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae ATP synthase subunit a (atpB), partial CSB-YP015070FNA1
CSB-EP015070FNA1
CSB-BP015070FNA1
CSB-MP015070FNA1
CSB-EP015070FNA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae GTPase Era (era) CSB-YP007759FNA
CSB-EP007759FNA
CSB-BP007759FNA
CSB-MP007759FNA
CSB-EP007759FNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Elongation factor Ts (tsf) CSB-YP025124FNA
CSB-EP025124FNA
CSB-BP025124FNA
CSB-MP025124FNA
CSB-EP025124FNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Translation initiation factor IF-2 (infB), partial CSB-YP015164FNA
CSB-EP015164FNA
CSB-BP015164FNA
CSB-MP015164FNA
CSB-EP015164FNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Elongation factor G (fusA), partial CSB-YP009373FNA
CSB-EP009373FNA
CSB-BP009373FNA
CSB-MP009373FNA
CSB-EP009373FNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Argininosuccinate synthase (argG) CSB-YP002234FNA
CSB-EP002234FNA
CSB-BP002234FNA
CSB-MP002234FNA
CSB-EP002234FNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulforudis audaxviator ATP synthase subunit delta (atpH) CSB-YP002355DJM
CSB-EP002355DJM
CSB-BP002355DJM
CSB-MP002355DJM
CSB-EP002355DJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulforudis audaxviator Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146DJM
CSB-EP019146DJM
CSB-BP019146DJM
CSB-MP019146DJM
CSB-EP019146DJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulforudis audaxviator Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799DJM
CSB-EP014799DJM
CSB-BP014799DJM
CSB-MP014799DJM
CSB-EP014799DJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Peptide chain release factor 1 (prfA) CSB-YP015197LRV
CSB-EP015197LRV
CSB-BP015197LRV
CSB-MP015197LRV
CSB-EP015197LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus ATP synthase subunit b (atpF), partial CSB-YP002358LRV1
CSB-EP002358LRV1
CSB-BP002358LRV1
CSB-MP002358LRV1
CSB-EP002358LRV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein ripply3 (dscr6) CSB-YP007198XBF
CSB-EP007198XBF
CSB-BP007198XBF
CSB-MP007198XBF
CSB-EP007198XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis E3 ubiquitin-protein ligase arih1 (arih1), partial CSB-YP002068XBF
CSB-EP002068XBF
CSB-BP002068XBF
CSB-MP002068XBF
CSB-EP002068XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>