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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Flagellar basal-body rod protein flgC (flgC) CSB-YP300290BIP
CSB-EP300290BIP
CSB-BP300290BIP
CSB-MP300290BIP
CSB-EP300290BIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S21 1 (rpsU1) CSB-YP300291BXW
CSB-EP300291BXW
CSB-BP300291BXW
CSB-MP300291BXW
CSB-EP300291BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transcriptional regulatory protein yccH (yccH) CSB-YP300292BRJ
CSB-EP300292BRJ
CSB-BP300292BRJ
CSB-MP300292BRJ
CSB-EP300292BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 (murAA) CSB-YP300293BRJ
CSB-EP300293BRJ
CSB-BP300293BRJ
CSB-MP300293BRJ
CSB-EP300293BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Modification methylase BsoBI (bsoBIM), partial CSB-YP300294GFM
CSB-EP300294GFM
CSB-BP300294GFM
CSB-MP300294GFM
CSB-EP300294GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Antilisterial bacteriocin subtilosin biosynthesis protein AlbE (albE) CSB-YP300295BRJ
CSB-EP300295BRJ
CSB-BP300295BRJ
CSB-MP300295BRJ
CSB-EP300295BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yuaB (yuaB), partial CSB-YP300296BRJ
CSB-BP300296BRJ
CSB-EP300296BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Small, acid-soluble spore protein O (sspO) CSB-YP300297BRJ
CSB-EP300297BRJ
CSB-BP300297BRJ
CSB-MP300297BRJ
CSB-EP300297BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional activator mta (mta) CSB-YP300298BRJ
CSB-EP300298BRJ
CSB-BP300298BRJ
CSB-MP300298BRJ
CSB-EP300298BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative HTH-type transcriptional regulator ywgB (ywgB) CSB-YP300299BRJ
CSB-EP300299BRJ
CSB-BP300299BRJ
CSB-MP300299BRJ
CSB-EP300299BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative glycosyltransferase epsH (epsH) CSB-YP300300BRJ
CSB-EP300300BRJ
CSB-BP300300BRJ
CSB-MP300300BRJ
CSB-EP300300BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yukC (yukC), partial CSB-YP300301BRJ1
CSB-EP300301BRJ1
CSB-BP300301BRJ1
CSB-MP300301BRJ1
CSB-EP300301BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative multidrug export ATP-binding/permease protein YgaD (ygaD), partial CSB-YP300302BRJ1
CSB-EP300302BRJ1
CSB-BP300302BRJ1
CSB-MP300302BRJ1
CSB-EP300302BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein traM (traM) CSB-YP300303ENL
CSB-EP300303ENL
CSB-BP300303ENL
CSB-MP300303ENL
CSB-EP300303ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Colanic acid biosynthesis protein wcaK (wcaK) CSB-YP300304ENV
CSB-EP300304ENV
CSB-BP300304ENV
CSB-MP300304ENV
CSB-EP300304ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized deacetylase yaiS (yaiS) CSB-YP300305ENV
CSB-EP300305ENV
CSB-BP300305ENV
CSB-MP300305ENV
CSB-EP300305ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Fe (3+)-transport system permease protein fbpB 2, partial CSB-YP300306HTA
CSB-EP300306HTA
CSB-BP300306HTA
CSB-MP300306HTA
CSB-EP300306HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Thiamine-phosphate synthase (thiE) CSB-YP300307HTA
CSB-EP300307HTA
CSB-BP300307HTA
CSB-MP300307HTA
CSB-EP300307HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Putative antitoxin RelB (relB) CSB-YP300308HTA
CSB-EP300308HTA
CSB-BP300308HTA
CSB-MP300308HTA
CSB-EP300308HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Uncharacterized permease HI_1015 (HI_1015), partial CSB-YP300309HTA
CSB-EP300309HTA
CSB-BP300309HTA
CSB-MP300309HTA
CSB-EP300309HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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