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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis Alpha N-terminal protein methyltransferase 1A (mettl11a) CSB-YP013725XBF
CSB-EP013725XBF
CSB-BP013725XBF
CSB-MP013725XBF
CSB-EP013725XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein kintoun (dnaaf2), partial CSB-YP003132XBE
CSB-EP003132XBE
CSB-BP003132XBE
CSB-MP003132XBE
CSB-EP003132XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Septin-8 (sept8) CSB-YP021032XBF
CSB-EP021032XBF
CSB-BP021032XBF
CSB-MP021032XBF
CSB-EP021032XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP015197UBJ
CSB-EP015197UBJ
CSB-BP015197UBJ
CSB-MP015197UBJ
CSB-EP015197UBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ureaplasma parvum serovar 3 Elongation factor G (fusA), partial CSB-YP009373UBK
CSB-EP009373UBK
CSB-BP009373UBK
CSB-MP009373UBK
CSB-EP009373UBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ureaplasma parvum serovar 3 ATP synthase subunit delta (atpH) CSB-YP002355UBK
CSB-EP002355UBK
CSB-BP002355UBK
CSB-MP002355UBK
CSB-EP002355UBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Interleukin-15 (IL15) CSB-YP011593AYX
CSB-EP011593AYX
CSB-BP011593AYX
CSB-MP011593AYX
CSB-EP011593AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Eukaryotic translation initiation factor 3 subunit J (hcr1) CSB-YP007539NGT
CSB-EP007539NGT
CSB-BP007539NGT
CSB-MP007539NGT
CSB-EP007539NGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Pescadillo homolog (nop7), partial CSB-YP017791NGT
CSB-EP017791NGT
CSB-BP017791NGT
CSB-MP017791NGT
CSB-EP017791NGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex quinquefasciatus Enolase-phosphatase E1 (CPIJ009638), partial CSB-YP007674DZM
CSB-EP007674DZM
CSB-BP007674DZM
CSB-MP007674DZM
CSB-EP007674DZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex quinquefasciatus DDRGK domain-containing protein 1 (CPIJ007479) CSB-YP006597DZM
CSB-EP006597DZM
CSB-BP006597DZM
CSB-MP006597DZM
CSB-EP006597DZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex quinquefasciatus Eukaryotic translation initiation factor 3 subunit B (eIF3-S9), partial CSB-YP007530DZM
CSB-EP007530DZM
CSB-BP007530DZM
CSB-MP007530DZM
CSB-EP007530DZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex quinquefasciatus Alpha-tubulin N-acetyltransferase (CPIJ002083) CSB-YP004059DZM
CSB-EP004059DZM
CSB-BP004059DZM
CSB-MP004059DZM
CSB-EP004059DZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex quinquefasciatus Adenylyltransferase and sulfurtransferase MOCS3 (CPIJ001621) CSB-YP014707DZM
CSB-EP014707DZM
CSB-BP014707DZM
CSB-MP014707DZM
CSB-EP014707DZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex quinquefasciatus Eukaryotic translation initiation factor 3 subunit G (eIF3-S4) CSB-YP007536DZM
CSB-EP007536DZM
CSB-BP007536DZM
CSB-MP007536DZM
CSB-EP007536DZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Elongation factor Ts (tsf) CSB-YP025124AWQ
CSB-EP025124AWQ
CSB-BP025124AWQ
CSB-MP025124AWQ
CSB-EP025124AWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146AWQ
CSB-EP019146AWQ
CSB-BP019146AWQ
CSB-MP019146AWQ
CSB-EP019146AWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Malate dehydrogenase (mdh) CSB-YP013623AWQ
CSB-EP013623AWQ
CSB-BP013623AWQ
CSB-MP013623AWQ
CSB-EP013623AWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717AWQ
CSB-EP009717AWQ
CSB-BP009717AWQ
CSB-MP009717AWQ
CSB-EP009717AWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ferrochelatase (hemH) CSB-YP008579AWQ
CSB-EP008579AWQ
CSB-BP008579AWQ
CSB-MP008579AWQ
CSB-EP008579AWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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