重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella suis biovar 1 Tryptophan--tRNA ligase (trpS) CSB-YP300678BMQ
CSB-EP300678BMQ
CSB-BP300678BMQ
CSB-MP300678BMQ
CSB-EP300678BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan--tRNA ligase (trpS) CSB-YP300679FMW
CSB-EP300679FMW
CSB-BP300679FMW
CSB-MP300679FMW
CSB-EP300679FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0267 protein yqfB (yqfB) CSB-YP300680ENV
CSB-EP300680ENV
CSB-BP300680ENV
CSB-MP300680ENV
CSB-EP300680ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine--tRNA ligase (tyrS) CSB-YP300681MVH
CSB-EP300681MVH
CSB-BP300681MVH
CSB-MP300681MVH
CSB-EP300681MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0270 protein YheU (yheU) CSB-YP300682ENV
CSB-EP300682ENV
CSB-BP300682ENV
CSB-MP300682ENV
CSB-EP300682ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine recombinase XerC (xerC) CSB-YP300683SKX
CSB-EP300683SKX
CSB-BP300683SKX
CSB-MP300683SKX
CSB-EP300683SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine recombinase XerD (xerD) CSB-YP300684MVH
CSB-EP300684MVH
CSB-BP300684MVH
CSB-MP300684MVH
CSB-EP300684MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fumarate reductase subunit D (frdD), partial CSB-YP300685MVZ1
CSB-EP300685MVZ1
CSB-BP300685MVZ1
CSB-MP300685MVZ1
CSB-EP300685MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP300686SWW
CSB-EP300686SWW
CSB-BP300686SWW
CSB-MP300686SWW
CSB-EP300686SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator yhaJ (yhaJ) CSB-YP300687ENV
CSB-EP300687ENV
CSB-BP300687ENV
CSB-MP300687ENV
CSB-EP300687ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Mb2303c (Mb2303c) CSB-YP300688MVH
CSB-EP300688MVH
CSB-BP300688MVH
CSB-MP300688MVH
CSB-EP300688MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Primosomal replication protein n (priB) CSB-YP300689SZB
CSB-EP300689SZB
CSB-BP300689SZB
CSB-MP300689SZB
CSB-EP300689SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Universal stress protein B (uspB), partial CSB-YP300690SWW1
CSB-EP300690SWW1
CSB-BP300690SWW1
CSB-MP300690SWW1
CSB-EP300690SWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Antitoxin HicB (hicB) CSB-YP300691ENV
CSB-EP300691ENV
CSB-BP300691ENV
CSB-MP300691ENV
CSB-EP300691ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Rv0023/MT0026 (Rv0023, MT0026) CSB-YP300692MVZ
CSB-EP300692MVZ
CSB-BP300692MVZ
CSB-MP300692MVZ
CSB-EP300692MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nucleoid-associated protein YejK (yejK) CSB-YP300693SWW
CSB-EP300693SWW
CSB-BP300693SWW
CSB-MP300693SWW
CSB-EP300693SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein sprT-like (SA1867) CSB-YP300694SKY
CSB-EP300694SKY
CSB-BP300694SKY
CSB-MP300694SKY
CSB-EP300694SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Transcriptional regulator NanR (nanR) CSB-YP300695SXB
CSB-EP300695SXB
CSB-BP300695SXB
CSB-MP300695SXB
CSB-EP300695SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Rv0586/MT0615 (Rv0586, MT0615) CSB-YP300696MVZ
CSB-EP300696MVZ
CSB-BP300696MVZ
CSB-MP300696MVZ
CSB-EP300696MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0301 protein Mb0039 (Mb0039) CSB-YP300697MVH
CSB-EP300697MVH
CSB-BP300697MVH
CSB-MP300697MVH
CSB-EP300697MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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