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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717FGA
CSB-EP009717FGA
CSB-BP009717FGA
CSB-MP009717FGA
CSB-EP009717FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Transaldolase (tal) CSB-YP023112FGA
CSB-EP023112FGA
CSB-BP023112FGA
CSB-MP023112FGA
CSB-EP023112FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Acyl carrier protein (acpP) CSB-YP015636FGA
CSB-EP015636FGA
CSB-BP015636FGA
CSB-MP015636FGA
CSB-EP015636FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Methionyl-tRNA formyltransferase (fmt) CSB-YP015150FGA
CSB-EP015150FGA
CSB-BP015150FGA
CSB-MP015150FGA
CSB-EP015150FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus Elongation factor G (fusA), partial CSB-YP009373TMI
CSB-EP009373TMI
CSB-BP009373TMI
CSB-MP009373TMI
CSB-EP009373TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus Peptide deformylase (def) CSB-YP017707TMI
CSB-EP017707TMI
CSB-BP017707TMI
CSB-MP017707TMI
CSB-EP017707TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus Thymidine kinase (tdk) CSB-YP023577TMI
CSB-EP023577TMI
CSB-BP023577TMI
CSB-MP023577TMI
CSB-EP023577TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter sp. tRNA pseudouridine synthase A (truA) CSB-YP019086TMJ
CSB-EP019086TMJ
CSB-BP019086TMJ
CSB-MP019086TMJ
CSB-EP019086TMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calmodulin-lysine N-methyltransferase (Camkmt) CSB-YP003841RA
CSB-EP003841RA
CSB-BP003841RA
CSB-MP003841RA
CSB-EP003841RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Cytosolic carboxypeptidase-like protein 5 (agbl5), partial CSB-YP001439XBF
CSB-EP001439XBF
CSB-BP001439XBF
CSB-MP001439XBF
CSB-EP001439XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcystis aeruginosa Ferrochelatase (hemH) CSB-YP008579MTQ
CSB-EP008579MTQ
CSB-BP008579MTQ
CSB-MP008579MTQ
CSB-EP008579MTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcystis aeruginosa Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824MTQ
CSB-EP005824MTQ
CSB-BP005824MTQ
CSB-MP005824MTQ
CSB-EP005824MTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum AP-1 complex subunit sigma-2 (ap1s2) CSB-YP001867DKK
CSB-EP001867DKK
CSB-BP001867DKK
CSB-MP001867DKK
CSB-EP001867DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aedes aegypti NADH-ubiquinone oxidoreductase chain 4L (mt:ND4L), partial CSB-YP015080AXQ1
CSB-EP015080AXQ1
CSB-BP015080AXQ1
CSB-MP015080AXQ1
CSB-EP015080AXQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laccaria bicolor Pescadillo homolog (NOP7), partial CSB-YP017791LLZ
CSB-EP017791LLZ
CSB-BP017791LLZ
CSB-MP017791LLZ
CSB-EP017791LLZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callithrix jacchus Insulin-induced gene 2 protein (INSIG2), partial CSB-YP011746CYL1
CSB-EP011746CYL1
CSB-BP011746CYL1
CSB-MP011746CYL1
CSB-EP011746CYL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Serine hydroxymethyltransferase (glyA) CSB-YP021274BWU
CSB-EP021274BWU
CSB-BP021274BWU
CSB-MP021274BWU
CSB-EP021274BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451BWU
CSB-EP015451BWU
CSB-BP015451BWU
CSB-MP015451BWU
CSB-EP015451BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Peptide chain release factor 1 (prfA) CSB-YP015197AVV
CSB-EP015197AVV
CSB-BP015197AVV
CSB-MP015197AVV
CSB-EP015197AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia rickettsii ATP synthase subunit a (atpB), partial CSB-YP015070RNB1
CSB-EP015070RNB1
CSB-BP015070RNB1
CSB-MP015070RNB1
CSB-EP015070RNB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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