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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vaccinia virus Protein F1 (F1L) CSB-YP300738VAD
CSB-EP300738VAD
CSB-BP300738VAD
CSB-MP300738VAD
CSB-EP300738VAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA-binding 11 kDa phosphoprotein (TF17R) CSB-YP300739VAH
CSB-EP300739VAH
CSB-BP300739VAH
CSB-MP300739VAH
CSB-EP300739VAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Telomere-binding protein I6 (I6L) CSB-YP300740VAA
CSB-EP300740VAA
CSB-BP300740VAA
CSB-MP300740VAA
CSB-EP300740VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 11.8 kDa protein (B ORF A) CSB-YP300741VAI
CSB-EP300741VAI
CSB-BP300741VAI
CSB-MP300741VAI
CSB-EP300741VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.9 kDa protein (D ORF D) CSB-YP300742VAI
CSB-EP300742VAI
CSB-BP300742VAI
CSB-MP300742VAI
CSB-EP300742VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.2 kDa protein (D ORF H) CSB-YP300743VAI
CSB-EP300743VAI
CSB-BP300743VAI
CSB-MP300743VAI
CSB-EP300743VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.6 kDa protein CSB-YP300744VAA
CSB-EP300744VAA
CSB-BP300744VAA
CSB-MP300744VAA
CSB-EP300744VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salvelinus alpinus Metallothionein B (mtb) CSB-YP300745SBW
CSB-EP300745SBW
CSB-BP300745SBW
CSB-MP300745SBW
CSB-EP300745SBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived protein nrdI (nrdIB) CSB-YP300746BRJ
CSB-EP300746BRJ
CSB-BP300746BRJ
CSB-MP300746BRJ
CSB-EP300746BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum ATP synthase protein MI25, partial CSB-YP300747TQN1
CSB-EP300747TQN1
CSB-BP300747TQN1
CSB-MP300747TQN1
CSB-EP300747TQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxoma virus MF7 protein (MVF7) CSB-YP300748MMT
CSB-EP300748MMT
CSB-BP300748MMT
CSB-MP300748MMT
CSB-EP300748MMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SPbeta Disulfide bond formation protein A (bdbA) CSB-YP300749BQZ
CSB-EP300749BQZ
CSB-BP300749BQZ
CSB-MP300749BQZ
CSB-EP300749BQZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived sublancin-168-processing and transport ATP-binding protein SunT (sunT), partial CSB-YP300750BRJ1
CSB-EP300750BRJ1
CSB-BP300750BRJ1
CSB-MP300750BRJ1
CSB-EP300750BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein psaE (psaE) CSB-YP300751YAH
CSB-EP300751YAH
CSB-BP300751YAH
CSB-MP300751YAH
CSB-EP300751YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus Protein A30 (A30L, A33L) CSB-YP300752VAR
CSB-EP300752VAR
CSB-BP300752VAR
CSB-MP300752VAR
CSB-EP300752VAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein F6 (F12L) CSB-YP300753VAD
CSB-EP300753VAD
CSB-BP300753VAD
CSB-MP300753VAD
CSB-EP300753VAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA-directed RNA polymerase 19 kDa subunit (RPO19) CSB-YP300754VAA
CSB-EP300754VAA
CSB-BP300754VAA
CSB-MP300754VAA
CSB-EP300754VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A33 (A33R), partial CSB-YP300755VAA1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Vaccinia virus Uncharacterized 9.9 kDa protein (SALLA) CSB-YP300756VAI
CSB-EP300756VAI
CSB-BP300756VAI
CSB-MP300756VAI
CSB-EP300756VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Viral replication protein A28 (VACWR151), partial CSB-YP300757VAI1
CSB-EP300757VAI1
CSB-BP300757VAI1
CSB-MP300757VAI1
CSB-EP300757VAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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