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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Equine herpesvirus 1 Envelope glycoprotein D (gD), partial CSB-YP301547EFN1
CSB-EP301547EFN1
CSB-BP301547EFN1
CSB-MP301547EFN1
CSB-EP301547EFN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tegument protein VP16 (UL48) CSB-YP301548HXA
CSB-EP301548HXA
CSB-BP301548HXA
CSB-MP301548HXA
CSB-EP301548HXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Uncharacterized gene 67 protein (IR6) CSB-YP301549EFM
CSB-EP301549EFM
CSB-BP301549EFM
CSB-MP301549EFM
CSB-EP301549EFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Tripartite terminase subunit UL28 homolog (32), partial CSB-YP301550EFL
CSB-EP301550EFL
CSB-BP301550EFL
CSB-MP301550EFL
CSB-EP301550EFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Necrosyrtes monachus Ovomucoid CSB-YP301551NBF
CSB-EP301551NBF
CSB-BP301551NBF
CSB-MP301551NBF
CSB-EP301551NBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Larus ridibundus Ovomucoid CSB-YP301552LCM
CSB-EP301552LCM
CSB-BP301552LCM
CSB-MP301552LCM
CSB-EP301552LCM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aythya americana Ovomucoid CSB-YP301553ATR
CSB-EP301553ATR
CSB-BP301553ATR
CSB-MP301553ATR
CSB-EP301553ATR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa velutina nigrithorax Melittin (MELT) CSB-YP301554VBU
CSB-EP301554VBU
CSB-BP301554VBU
CSB-MP301554VBU
CSB-EP301554VBU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja nivea Short neurotoxin 1 CSB-YP301555NAK
CSB-EP301555NAK
CSB-BP301555NAK
CSB-MP301555NAK
CSB-EP301555NAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris ocellata Ovomucoid CSB-YP301556MDN
CSB-EP301556MDN
CSB-BP301556MDN
CSB-MP301556MDN
CSB-EP301556MDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein B6 (B6R) CSB-YP301557VAA
CSB-EP301557VAA
CSB-BP301557VAA
CSB-MP301557VAA
CSB-EP301557VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein F1 (F1L) CSB-YP301558VAA
CSB-EP301558VAA
CSB-BP301558VAA
CSB-MP301558VAA
CSB-EP301558VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein G3 (G3L), partial CSB-YP301559VAA
CSB-EP301559VAA
CSB-BP301559VAA
CSB-MP301559VAA
CSB-EP301559VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein K7 (K7R) CSB-YP301560VAA
CSB-EP301560VAA
CSB-BP301560VAA
CSB-MP301560VAA
CSB-EP301560VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 11.8 kDa protein (B ORF A) CSB-YP301561VAA
CSB-EP301561VAA
CSB-BP301561VAA
CSB-MP301561VAA
CSB-EP301561VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.3 kDa protein (D ORF E) CSB-YP301562VAA
CSB-EP301562VAA
CSB-BP301562VAA
CSB-MP301562VAA
CSB-EP301562VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.0 kDa protein CSB-YP301563VAA
CSB-EP301563VAA
CSB-BP301563VAA
CSB-MP301563VAA
CSB-EP301563VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo Metallothionein CSB-YP301564MQV
CSB-EP301564MQV
CSB-BP301564MQV
CSB-MP301564MQV
CSB-EP301564MQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus Uncharacterized mitochondrial protein ORF138, partial CSB-YP301565BWD1
CSB-EP301565BWD1
CSB-BP301565BWD1
CSB-MP301565BWD1
CSB-EP301565BWD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camelpox virus Viral replication protein A28-like (CMP145L), partial CSB-YP301568CBR
CSB-EP301568CBR
CSB-BP301568CBR
CSB-MP301568CBR
CSB-EP301568CBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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