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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP300982SSQ
CSB-EP300982SSQ
CSB-BP300982SSQ
CSB-MP300982SSQ
CSB-EP300982SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phosphate transport system protein phoU homolog (phoU) CSB-YP300983SSQ
CSB-EP300983SSQ
CSB-BP300983SSQ
CSB-MP300983SSQ
CSB-EP300983SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein slr0245 (slr0245) CSB-YP300984SSQ
CSB-EP300984SSQ
CSB-BP300984SSQ
CSB-MP300984SSQ
CSB-EP300984SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative diflavin flavoprotein A 4 (dfa4), partial CSB-YP300985SSQ
CSB-EP300985SSQ
CSB-BP300985SSQ
CSB-MP300985SSQ
CSB-EP300985SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable aromatic acid decarboxylase (slr1099) CSB-YP300986SSQ
CSB-EP300986SSQ
CSB-BP300986SSQ
CSB-MP300986SSQ
CSB-EP300986SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Carbon dioxide-concentrating mechanism protein CcmK homolog 1 (ccmK1) CSB-YP300987SSQ
CSB-EP300987SSQ
CSB-BP300987SSQ
CSB-MP300987SSQ
CSB-EP300987SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative serine protease HhoA (hhoA) CSB-YP300988SSQ
CSB-EP300988SSQ
CSB-BP300988SSQ
CSB-MP300988SSQ
CSB-EP300988SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Peptide methionine sulfoxide reductase MsrA 2 (msrA2) CSB-YP300989SSQ
CSB-EP300989SSQ
CSB-BP300989SSQ
CSB-MP300989SSQ
CSB-EP300989SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP300990SSQ1
CSB-EP300990SSQ1
CSB-BP300990SSQ1
CSB-MP300990SSQ1
CSB-EP300990SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 30S ribosomal protein S15 (rpsO) CSB-YP300991SSQ
CSB-EP300991SSQ
CSB-BP300991SSQ
CSB-MP300991SSQ
CSB-EP300991SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0150 protein ssr1765 (ssr1765) CSB-YP300992SSQ
CSB-EP300992SSQ
CSB-BP300992SSQ
CSB-MP300992SSQ
CSB-EP300992SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative ammonium transporter sll1017 (sll1017), partial CSB-YP300993SSQ
CSB-EP300993SSQ
CSB-BP300993SSQ
CSB-MP300993SSQ
CSB-EP300993SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Thiamine-phosphate synthase (thiE) CSB-YP300994SSQ
CSB-EP300994SSQ
CSB-BP300994SSQ
CSB-MP300994SSQ
CSB-EP300994SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem I reaction center subunit XII (psaM), partial CSB-YP300995SSQ
CSB-EP300995SSQ
CSB-BP300995SSQ
CSB-MP300995SSQ
CSB-EP300995SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 50S ribosomal protein L32 (rpmF) CSB-YP300996SSQ
CSB-EP300996SSQ
CSB-BP300996SSQ
CSB-MP300996SSQ
CSB-EP300996SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Urease accessory protein UreD (ureD) CSB-YP300997SSQ
CSB-EP300997SSQ
CSB-BP300997SSQ
CSB-MP300997SSQ
CSB-EP300997SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Rubredoxin (rub) CSB-YP300998SSQ
CSB-EP300998SSQ
CSB-BP300998SSQ
CSB-MP300998SSQ
CSB-EP300998SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. DNA topoisomerase 4 subunit A (parC), partial CSB-YP300999SSQ
CSB-EP300999SSQ
CSB-BP300999SSQ
CSB-MP300999SSQ
CSB-EP300999SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable RuBisCO transcriptional regulator (rbcR) CSB-YP301000SSQ
CSB-EP301000SSQ
CSB-BP301000SSQ
CSB-MP301000SSQ
CSB-EP301000SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Tyrosine--tRNA ligase (tyrS) CSB-YP301001SSQ
CSB-EP301001SSQ
CSB-BP301001SSQ
CSB-MP301001SSQ
CSB-EP301001SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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