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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yop proteins translocation protein I (yscI) CSB-YP301731YAS
CSB-EP301731YAS
CSB-BP301731YAS
CSB-MP301731YAS
CSB-EP301731YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation protein M (yscM) CSB-YP301732YAS
CSB-EP301732YAS
CSB-BP301732YAS
CSB-MP301732YAS
CSB-EP301732YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation protein T (yscT), partial CSB-YP301733YAH1
CSB-EP301733YAH1
CSB-BP301733YAH1
CSB-MP301733YAH1
CSB-EP301733YAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease accessory protein UreG (ureG) CSB-YP301734YAS
CSB-EP301734YAS
CSB-BP301734YAS
CSB-MP301734YAS
CSB-EP301734YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis GATA-binding factor 6-B (gata6-b) CSB-YP301735XBE
CSB-EP301735XBE
CSB-BP301735XBE
CSB-MP301735XBE
CSB-EP301735XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Histone H4 type VIII (H4-VIII) CSB-YP301736CH
CSB-EP301736CH
CSB-BP301736CH
CSB-MP301736CH
CSB-EP301736CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone H2A.Z-like (h2a.zl1) CSB-YP301737XBE
CSB-EP301737XBE
CSB-BP301737XBE
CSB-MP301737XBE
CSB-EP301737XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Homeobox protein MSX-3 (Msx3) CSB-YP301738MO
CSB-EP301738MO
CSB-BP301738MO
CSB-MP301738MO
CSB-EP301738MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Testis-specific protein TSX (Tsx) CSB-YP301739MO
CSB-EP301739MO
CSB-BP301739MO
CSB-MP301739MO
CSB-EP301739MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ubiquitin-conjugating enzyme E2 D2B (Ube2d2b) CSB-YP301740RA
CSB-EP301740RA
CSB-BP301740RA
CSB-MP301740RA
CSB-EP301740RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein MutS (mutS), partial CSB-YP301741DNW
CSB-EP301741DNW
CSB-BP301741DNW
CSB-MP301741DNW
CSB-EP301741DNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis HTH-type transcriptional regulator ttuA (ttuA) CSB-YP301742AYT
CSB-EP301742AYT
CSB-BP301742AYT
CSB-MP301742AYT
CSB-EP301742AYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alginate biosynthesis protein AlgF (algF) CSB-YP301744DPX
CSB-EP301744DPX
CSB-BP301744DPX
CSB-MP301744DPX
CSB-EP301744DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Uncharacterized protein BBD21 (BB_D21) CSB-YP301745BUD
CSB-EP301745BUD
CSB-BP301745BUD
CSB-MP301745BUD
CSB-EP301745BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta- (1-->2)glucan export ATP-binding/permease protein NdvA, partial CSB-YP301746BSE1
CSB-EP301746BSE1
CSB-BP301746BSE1
CSB-MP301746BSE1
CSB-EP301746BSE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variable large protein 5 (vlp5) CSB-YP301747BIP
CSB-EP301747BIP
CSB-BP301747BIP
CSB-MP301747BIP
CSB-EP301747BIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variable large protein 23 (vlp23) CSB-YP301748BIP
CSB-EP301748BIP
CSB-BP301748BIP
CSB-MP301748BIP
CSB-EP301748BIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative phosphatase YitU (yitU) CSB-YP301749BRJ
CSB-EP301749BRJ
CSB-BP301749BRJ
CSB-MP301749BRJ
CSB-EP301749BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywmD (ywmD) CSB-YP301750BRJ
CSB-EP301750BRJ
CSB-BP301750BRJ
CSB-MP301750BRJ
CSB-EP301750BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Energy-coupling factor transporter transmembrane protein EcfT (ecfT), partial CSB-YP301751BRJ1
CSB-EP301751BRJ1
CSB-BP301751BRJ1
CSB-MP301751BRJ1
CSB-EP301751BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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