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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Myxoma virus Serine proteinase inhibitor 2 (SERP2) CSB-YP301569MMS
CSB-EP301569MMS
CSB-BP301569MMS
CSB-MP301569MMS
CSB-EP301569MMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SPbeta Disulfide bond formation protein B (bdbB), partial CSB-YP301570BQZ1
CSB-EP301570BQZ1
CSB-BP301570BQZ1
CSB-MP301570BQZ1
CSB-EP301570BQZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SPbeta Bacteriocin sublancin-168 (sunA) CSB-YP301571BQZ
CSB-EP301571BQZ
CSB-BP301571BQZ
CSB-MP301571BQZ
CSB-EP301571BQZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Virion membrane protein A17 precursor (VACWR137), partial CSB-YP301572VAI1
CSB-EP301572VAI1
CSB-BP301572VAI1
CSB-MP301572VAI1
CSB-EP301572VAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Interferon antagonist C7L (VACWR021) CSB-YP301573VAI
CSB-EP301573VAI
CSB-BP301573VAI
CSB-MP301573VAI
CSB-EP301573VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA-directed RNA polymerase 22 kDa subunit (RPO22) CSB-YP301574VAI
CSB-EP301574VAI
CSB-BP301574VAI
CSB-MP301574VAI
CSB-EP301574VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A36 (A36R), partial CSB-YP301575VAA
CSB-EP301575VAA
CSB-BP301575VAA
CSB-MP301575VAA
CSB-EP301575VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.6 kDa protein (A ORF M) CSB-YP301576VAA
CSB-EP301576VAA
CSB-BP301576VAA
CSB-MP301576VAA
CSB-EP301576VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein fixC (fixC) CSB-YP301578ENV
CSB-EP301578ENV
CSB-BP301578ENV
CSB-MP301578ENV
CSB-EP301578ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-like protein fixX (fixX) CSB-YP301579EOD
CSB-EP301579EOD
CSB-BP301579EOD
CSB-MP301579EOD
CSB-EP301579EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P21 Terminase small subunit (1) CSB-YP301580EDF
CSB-EP301580EDF
CSB-BP301580EDF
CSB-MP301580EDF
CSB-EP301580EDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 82 prophage-derived uncharacterized protein ybcO (ybcO) CSB-YP301581ENV
CSB-EP301581ENV
CSB-BP301581ENV
CSB-MP301581ENV
CSB-EP301581ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas phage phiLf Tail virion protein G9P (IX), partial CSB-YP301582XBD
CSB-EP301582XBD
CSB-BP301582XBD
CSB-MP301582XBD
CSB-EP301582XBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas phage phiLf DNA-Binding protein G5P (V) CSB-YP301583XBD
CSB-EP301583XBD
CSB-BP301583XBD
CSB-MP301583XBD
CSB-EP301583XBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S21 (rpsU) CSB-YP301584SWW
CSB-EP301584SWW
CSB-BP301584SWW
CSB-MP301584SWW
CSB-EP301584SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA topoisomerase 1B (TOP1) CSB-YP301585VAI
CSB-EP301585VAI
CSB-BP301585VAI
CSB-MP301585VAI
CSB-EP301585VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c (atpE), partial CSB-YP301586YAS1
CSB-EP301586YAS1
CSB-BP301586YAS1
CSB-MP301586YAS1
CSB-EP301586YAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A19 (VACWR139) CSB-YP301587VAI
CSB-EP301587VAI
CSB-BP301587VAI
CSB-MP301587VAI
CSB-EP301587VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Intermediate transcription factor 3 small subunit (VITF3S) CSB-YP301588VAI
CSB-EP301588VAI
CSB-BP301588VAI
CSB-MP301588VAI
CSB-EP301588VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Insect toxin 2-13 CSB-YP301589LDS
CSB-EP301589LDS
CSB-BP301589LDS
CSB-MP301589LDS
CSB-EP301589LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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