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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Ammonia channel (amtB), partial CSB-YP303180ENV
CSB-EP303180ENV
CSB-BP303180ENV
CSB-MP303180ENV
CSB-EP303180ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta vulgaris Photosystem II reaction center protein J (psbJ), partial CSB-YP303181BSQ
CSB-EP303181BSQ
CSB-BP303181BSQ
CSB-MP303181BSQ
CSB-EP303181BSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat CSB-YP303182HKQ
CSB-EP303182HKQ
CSB-BP303182HKQ
CSB-MP303182HKQ
CSB-EP303182HKQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Membrane protein (M), partial CSB-YP303183BJN1
CSB-EP303183BJN1
CSB-BP303183BJN1
CSB-MP303183BJN1
CSB-EP303183BJN1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Woodchuck hepatitis B virus Capsid protein (C) CSB-YP303184WAU
CSB-EP303184WAU
CSB-BP303184WAU
CSB-MP303184WAU
CSB-EP303184WAU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Vpr (vpr) CSB-YP303186HLA
CSB-EP303186HLA
CSB-BP303186HLA
CSB-MP303186HLA
CSB-EP303186HLA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus lynceus Conantokin-L CSB-YP303187COR
CSB-EP303187COR
CSB-BP303187COR
CSB-MP303187COR
CSB-EP303187COR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus catus Delta-conotoxin-like CVIE CSB-YP303188COE
CSB-EP303188COE
CSB-BP303188COE
CSB-MP303188COE
CSB-EP303188COE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus victoriae Conotoxin Vc6a CSB-YP303189CPN
CSB-EP303189CPN
CSB-BP303189CPN
CSB-MP303189CPN
CSB-EP303189CPN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus victoriae Conotoxin vc5a CSB-YP303190CPN
CSB-EP303190CPN
CSB-BP303190CPN
CSB-MP303190CPN
CSB-EP303190CPN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable aromatic acid decarboxylase (pad1) CSB-YP303191EOD
CSB-EP303191EOD
CSB-BP303191EOD
CSB-MP303191EOD
CSB-EP303191EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major outer membrane lipoprotein (lpp) CSB-YP303192EGX
CSB-EP303192EGX
CSB-BP303192EGX
CSB-MP303192EGX
CSB-EP303192EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli PTS system glucose-specific EIICB component (ptsG), partial CSB-YP303193ENV
CSB-EP303193ENV
CSB-BP303193ENV
CSB-MP303193ENV
CSB-EP303193ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PTS system mannose-specific EIIAB component (manX) CSB-YP303195SZB
CSB-EP303195SZB
CSB-BP303195SZB
CSB-MP303195SZB
CSB-EP303195SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fructose-like phosphotransferase enzyme IIB component 1 (fryB) CSB-YP303196ENV
CSB-EP303196ENV
CSB-BP303196ENV
CSB-MP303196ENV
CSB-EP303196ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogen regulatory protein (ptsN) CSB-YP303197SZB
CSB-EP303197SZB
CSB-BP303197SZB
CSB-MP303197SZB
CSB-EP303197SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascorbate-specific phosphotransferase enzyme IIB component (ulaB) CSB-YP303198EOD
CSB-EP303198EOD
CSB-BP303198EOD
CSB-MP303198EOD
CSB-EP303198EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Galactitol permease IIC component (gatC), partial CSB-YP303199ENV
CSB-EP303199ENV
CSB-BP303199ENV
CSB-MP303199ENV
CSB-EP303199ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pogonoperca punctata Grammistin Pp 4a CSB-YP303200PNY
CSB-EP303200PNY
CSB-BP303200PNY
CSB-MP303200PNY
CSB-EP303200PNY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Grammistes sexlineatus Grammistin Gs A CSB-YP303201GDD
CSB-EP303201GDD
CSB-BP303201GDD
CSB-MP303201GDD
CSB-EP303201GDD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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