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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Asparagine--tRNA ligase (asnS) CSB-YP303832SKX
CSB-EP303832SKX
CSB-BP303832SKX
CSB-MP303832SKX
CSB-EP303832SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Threonine--tRNA ligase (thrS), partial CSB-YP303833MVZ
CSB-EP303833MVZ
CSB-BP303833MVZ
CSB-MP303833MVZ
CSB-EP303833MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tryptophan--tRNA ligase (trpS) CSB-YP303834SUL
CSB-EP303834SUL
CSB-BP303834SUL
CSB-MP303834SUL
CSB-EP303834SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0266 membrane protein yobD (yobD), partial CSB-YP303835EGX1
CSB-EP303835EGX1
CSB-BP303835EGX1
CSB-MP303835EGX1
CSB-EP303835EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0265 protein yeeX (yeeX) CSB-YP303836SWW
CSB-EP303836SWW
CSB-BP303836SWW
CSB-MP303836SWW
CSB-EP303836SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP303837SWW
CSB-EP303837SWW
CSB-BP303837SWW
CSB-MP303837SWW
CSB-EP303837SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcription factor fapR (fapR) CSB-YP303838SUL
CSB-EP303838SUL
CSB-BP303838SUL
CSB-MP303838SUL
CSB-EP303838SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine recombinase XerS (xerS) CSB-YP303839SMH
CSB-EP303839SMH
CSB-BP303839SMH
CSB-MP303839SMH
CSB-EP303839SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0735 ACT domain-containing protein SA1469 (SA1469) CSB-YP303840SKY
CSB-EP303840SKY
CSB-BP303840SKY
CSB-MP303840SKY
CSB-EP303840SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP303841SXB
CSB-EP303841SXB
CSB-BP303841SXB
CSB-MP303841SXB
CSB-EP303841SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant MTA/SAH nucleosidase (mtnN) CSB-YP303842MVH
CSB-EP303842MVH
CSB-BP303842MVH
CSB-MP303842MVH
CSB-EP303842MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Rv2282c/MT2340 (Rv2282c, MT2340) CSB-YP303843MVZ
CSB-EP303843MVZ
CSB-BP303843MVZ
CSB-MP303843MVZ
CSB-EP303843MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Primosomal replication protein n (priB) CSB-YP303844BIK
CSB-EP303844BIK
CSB-BP303844BIK
CSB-MP303844BIK
CSB-EP303844BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP303845SKY
CSB-EP303845SKY
CSB-BP303845SKY
CSB-MP303845SKY
CSB-EP303845SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0289 protein BB4433 (BB4433) CSB-YP303846BTY
CSB-EP303846BTY
CSB-BP303846BTY
CSB-MP303846BTY
CSB-EP303846BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator YddM (yddM) CSB-YP303847ENV
CSB-EP303847ENV
CSB-BP303847ENV
CSB-MP303847ENV
CSB-EP303847ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Flagellar hook-basal body complex protein FliE (fliE) CSB-YP303848BMQ
CSB-EP303848BMQ
CSB-BP303848BMQ
CSB-MP303848BMQ
CSB-EP303848BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable tRNA-dihydrouridine synthase (dus) CSB-YP303849SKY
CSB-EP303849SKY
CSB-BP303849SKY
CSB-MP303849SKY
CSB-EP303849SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidinol-phosphate aminotransferase (hisC) CSB-YP303850SKX
CSB-EP303850SKX
CSB-BP303850SKX
CSB-MP303850SKX
CSB-EP303850SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC) CSB-YP303851MVZ
CSB-EP303851MVZ
CSB-BP303851MVZ
CSB-MP303851MVZ
CSB-EP303851MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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