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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cyanochen cyanoptera Ovomucoid CSB-YP303872CUX
CSB-EP303872CUX
CSB-BP303872CUX
CSB-MP303872CUX
CSB-EP303872CUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sarkidiornis melanotos Ovomucoid CSB-YP303873SCU
CSB-EP303873SCU
CSB-BP303873SCU
CSB-MP303873SCU
CSB-EP303873SCU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Datura stramonium Ferredoxin CSB-YP303874DAW
CSB-EP303874DAW
CSB-BP303874DAW
CSB-MP303874DAW
CSB-EP303874DAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strictosidine synthase (STR1) CSB-YP303875RBX
CSB-EP303875RBX
CSB-BP303875RBX
CSB-MP303875RBX
CSB-EP303875RBX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maltose/maltodextrin import ATP-binding protein MalK (malK) CSB-YP303876ENV
CSB-EP303876ENV
CSB-BP303876ENV
CSB-MP303876ENV
CSB-EP303876ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Troponin I, fast skeletal muscle (TNNI2) CSB-YP303877DXJ
CSB-EP303877DXJ
CSB-BP303877DXJ
CSB-MP303877DXJ
CSB-EP303877DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methicillin resistance regulatory protein mecI (mecI) CSB-YP303878FKZ
CSB-EP303878FKZ
CSB-BP303878FKZ
CSB-MP303878FKZ
CSB-EP303878FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Erythrodihydroneopterin triphosphate synthetase CSB-YP303879RA
CSB-EP303879RA
CSB-BP303879RA
CSB-MP303879RA
CSB-EP303879RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Viral late gene transcription factor 3 (VLTF3) CSB-YP303880VAA
CSB-EP303880VAA
CSB-BP303880VAA
CSB-MP303880VAA
CSB-EP303880VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Envelope glycoprotein I (GI), partial CSB-YP303881EFN1
CSB-EP303881EFN1
CSB-BP303881EFN1
CSB-MP303881EFN1
CSB-EP303881EFN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tegument protein VP16 (UL48) CSB-YP303882HJX
CSB-EP303882HJX
CSB-BP303882HJX
CSB-MP303882HJX
CSB-EP303882HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Envelope glycoprotein GP350 (BLLF1), partial CSB-YP303883EEZ
CSB-EP303883EEZ
CSB-BP303883EEZ
CSB-MP303883EEZ
CSB-EP303883EEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Grus vipio Ovomucoid CSB-YP303884GDQ
CSB-EP303884GDQ
CSB-BP303884GDQ
CSB-MP303884GDQ
CSB-EP303884GDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Netta rufina Ovomucoid CSB-YP303885NCI
CSB-EP303885NCI
CSB-BP303885NCI
CSB-MP303885NCI
CSB-EP303885NCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrophis ornatus Short neurotoxin 1 CSB-YP303886HRB
CSB-EP303886HRB
CSB-BP303886HRB
CSB-MP303886HRB
CSB-EP303886HRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti U3-theraphotoxin-Hh1a CSB-YP303887HUL
CSB-EP303887HUL
CSB-BP303887HUL
CSB-MP303887HUL
CSB-EP303887HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial CSB-YP303888VAA
CSB-BP303888VAA
CSB-MP303888VAA
CSB-EP303888VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein E7 (VACWR063) CSB-YP303889VAI
CSB-EP303889VAI
CSB-BP303889VAI
CSB-MP303889VAI
CSB-EP303889VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein F9 (F9), partial CSB-YP303890VAD1
CSB-EP303890VAD1
CSB-BP303890VAD1
CSB-MP303890VAD1
CSB-EP303890VAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein G3 (VACWR079), partial CSB-YP303891VAI
CSB-EP303891VAI
CSB-BP303891VAI
CSB-MP303891VAI
CSB-EP303891VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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