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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Schizosaccharomyces pombe Tip elongation protein 1 (tip1) CSB-YP305247SXV
CSB-EP305247SXV
CSB-BP305247SXV
CSB-MP305247SXV
CSB-EP305247SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe N-acetyltransferase ats1 (ats1) CSB-YP305248SXV
CSB-EP305248SXV
CSB-BP305248SXV
CSB-MP305248SXV
CSB-EP305248SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Interleukin-6 (IL6) CSB-YP305249MOV
CSB-EP305249MOV
CSB-BP305249MOV
CSB-MP305249MOV
CSB-EP305249MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Metallothionein-2A (MT2A) CSB-YP305250PI
CSB-EP305250PI
CSB-BP305250PI
CSB-MP305250PI
CSB-EP305250PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage Mu Tail sheath protein (L) CSB-YP305251EDA
CSB-EP305251EDA
CSB-BP305251EDA
CSB-MP305251EDA
CSB-EP305251EDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Tiggy-winkle hedgehog protein (twhh) CSB-YP305252DQG
CSB-EP305252DQG
CSB-BP305252DQG
CSB-MP305252DQG
CSB-EP305252DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio POU domain, class 3, transcription factor 3-B (pou3f3b) CSB-YP305253DIL
CSB-EP305253DIL
CSB-BP305253DIL
CSB-MP305253DIL
CSB-EP305253DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Complement component C9 (c9), partial CSB-YP305254TKY
CSB-EP305254TKY
CSB-BP305254TKY
CSB-MP305254TKY
CSB-EP305254TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana temporaria Temporin-G CSB-YP305255RBJ
CSB-EP305255RBJ
CSB-BP305255RBJ
CSB-MP305255RBJ
CSB-EP305255RBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Beta-1,3-N-acetylglucosaminyltransferase radical fringe (rfng), partial CSB-YP305256XBE
CSB-EP305256XBE
CSB-BP305256XBE
CSB-MP305256XBE
CSB-EP305256XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caudina arenicola Globin D, coelomic CSB-YP305257CEX
CSB-EP305257CEX
CSB-BP305257CEX
CSB-MP305257CEX
CSB-EP305257CEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus gammarus Crustacyanin-C1 subunit CSB-YP305258HHQ
CSB-EP305258HHQ
CSB-BP305258HHQ
CSB-MP305258HHQ
CSB-EP305258HHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsicum annuum Geranylgeranyl pyrophosphate synthase, chloroplastic (GGPS1), partial CSB-YP305259DQD
CSB-EP305259DQD
CSB-BP305259DQD
CSB-MP305259DQD
CSB-EP305259DQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dioscorea japonica Acidic endochitinase CSB-YP305260DEK
CSB-EP305260DEK
CSB-BP305260DEK
CSB-MP305260DEK
CSB-EP305260DEK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Pars intercerebralis major peptide D1 CSB-YP305261LHY
CSB-EP305261LHY
CSB-BP305261LHY
CSB-MP305261LHY
CSB-EP305261LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylaspartate mutase E chain (glmE) CSB-YP305262CLT
CSB-EP305262CLT
CSB-BP305262CLT
CSB-MP305262CLT
CSB-EP305262CLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymnaea stagnalis Molluscan insulin-related peptide 3 CSB-YP305263LRQ
CSB-EP305263LRQ
CSB-BP305263LRQ
CSB-MP305263LRQ
CSB-EP305263LRQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima 4-alpha-glucanotransferase (mgtA) CSB-YP305264TNJ
CSB-EP305264TNJ
CSB-BP305264TNJ
CSB-MP305264TNJ
CSB-EP305264TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Light-harvesting protein B800/850/890 beta-1 chain, partial CSB-YP305265HSG
CSB-EP305265HSG
CSB-BP305265HSG
CSB-MP305265HSG
CSB-EP305265HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein hrpB3 (hrpB3) CSB-YP305266XAK
CSB-EP305266XAK
CSB-BP305266XAK
CSB-MP305266XAK
CSB-EP305266XAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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