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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas aeruginosa D-alanyl-D-alanine endopeptidase (pbpG) CSB-YP304116EZX
CSB-EP304116EZX
CSB-BP304116EZX
CSB-MP304116EZX
CSB-EP304116EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S13 (rpsM) CSB-YP304117PBZ
CSB-EP304117PBZ
CSB-BP304117PBZ
CSB-MP304117PBZ
CSB-EP304117PBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Temperature acclimation protein A (tapA) CSB-YP304118PUK
CSB-EP304118PUK
CSB-BP304118PUK
CSB-MP304118PUK
CSB-EP304118PUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica Biopolymer transport protein exbD (exbD), partial CSB-YP304119ESE
CSB-EP304119ESE
CSB-BP304119ESE
CSB-MP304119ESE
CSB-EP304119ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (bphB) CSB-YP304120FFZ
CSB-EP304120FFZ
CSB-BP304120FFZ
CSB-MP304120FFZ
CSB-EP304120FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pimelobacter sp. Trehalose synthase (treS), partial CSB-YP304121PKA
CSB-EP304121PKA
CSB-BP304121PKA
CSB-MP304121PKA
CSB-EP304121PKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium fredii Uncharacterized protein ORF7 CSB-YP304122RKM
CSB-EP304122RKM
CSB-BP304122RKM
CSB-MP304122RKM
CSB-EP304122RKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Octopine transport system permease protein occQ (occQ), partial CSB-YP304123RKT1
CSB-EP304123RKT1
CSB-BP304123RKT1
CSB-MP304123RKT1
CSB-EP304123RKT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkyl hydroperoxide reductase subunit C (ahpC) CSB-YP304124RMA
CSB-EP304124RMA
CSB-BP304124RMA
CSB-MP304124RMA
CSB-EP304124RMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nod factor export ATP-binding protein I (nodI) CSB-YP304125RCY
CSB-EP304125RCY
CSB-BP304125RCY
CSB-MP304125RCY
CSB-EP304125RCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Antiholin-like protein LrgA (lrgA), partial CSB-YP304126FLF1
CSB-EP304126FLF1
CSB-BP304126FLF1
CSB-MP304126FLF1
CSB-EP304126FLF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor 30S ribosomal protein S11 (rpsK) CSB-YP304127FOB
CSB-EP304127FOB
CSB-BP304127FOB
CSB-MP304127FOB
CSB-EP304127FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP304128FMR1
CSB-EP304128FMR1
CSB-BP304128FMR1
CSB-MP304128FMR1
CSB-EP304128FMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 4 Competence-stimulating peptide type 2 (comC2) CSB-YP304129FMW
CSB-EP304129FMW
CSB-BP304129FMW
CSB-MP304129FMW
CSB-EP304129FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracycline resistance protein tetO (tetO), partial CSB-YP304130FMW
CSB-EP304130FMW
CSB-BP304130FMW
CSB-MP304130FMW
CSB-EP304130FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Type 4 prepilin-like proteins leader peptide-processing enzyme (hofD), partial CSB-YP304131SSQ1
CSB-EP304131SSQ1
CSB-BP304131SSQ1
CSB-MP304131SSQ1
CSB-EP304131SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP304132SSQ
CSB-EP304132SSQ
CSB-BP304132SSQ
CSB-MP304132SSQ
CSB-EP304132SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0045 protein sll0230 (sll0230) CSB-YP304133SSQ
CSB-EP304133SSQ
CSB-BP304133SSQ
CSB-MP304133SSQ
CSB-EP304133SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Cobalt-precorrin-6A reductase (cbiJ) CSB-YP304134SSQ
CSB-EP304134SSQ
CSB-BP304134SSQ
CSB-MP304134SSQ
CSB-EP304134SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0272 protein slr1411 (slr1411) CSB-YP304135SSQ
CSB-EP304135SSQ
CSB-BP304135SSQ
CSB-MP304135SSQ
CSB-EP304135SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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