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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Haemophilus influenzae Putative binding protein HI_1525 (HI_1525) CSB-YP304096HTA
CSB-EP304096HTA
CSB-BP304096HTA
CSB-MP304096HTA
CSB-EP304096HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Chemotaxis protein CheY homolog (cheY) CSB-YP304097HUV
CSB-EP304097HUV
CSB-BP304097HUV
CSB-MP304097HUV
CSB-EP304097HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Beta-phosphoglucomutase (pgmB) CSB-YP304098LNG
CSB-EP304098LNG
CSB-BP304098LNG
CSB-MP304098LNG
CSB-EP304098LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum Protein grpE (grpE) CSB-YP304099MLJ
CSB-EP304099MLJ
CSB-BP304099MLJ
CSB-MP304099MLJ
CSB-EP304099MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis 3-oxoacyl-[acyl-carrier-protein] reductase FabG (fabG) CSB-YP304100MVX
CSB-EP304100MVX
CSB-BP304100MVX
CSB-MP304100MVX
CSB-EP304100MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Succinyl-CoA ligase [ADP-forming] subunit alpha (sucD) CSB-YP304101MVZ
CSB-EP304101MVZ
CSB-BP304101MVZ
CSB-MP304101MVZ
CSB-EP304101MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv0007/MT0007 (Rv0007, MT0007), partial CSB-YP304102MVZ1
CSB-EP304102MVZ1
CSB-BP304102MVZ1
CSB-MP304102MVZ1
CSB-EP304102MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease VapC22 (vapC22) CSB-YP304103MVZ
CSB-EP304103MVZ
CSB-BP304103MVZ
CSB-MP304103MVZ
CSB-EP304103MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0052 protein Rv1422/MT1465 (Rv1422, MT1465) CSB-YP304104MVZ
CSB-EP304104MVZ
CSB-BP304104MVZ
CSB-MP304104MVZ
CSB-EP304104MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enhanced intracellular survival protein (eis) CSB-YP304105MVZ
CSB-EP304105MVZ
CSB-BP304105MVZ
CSB-MP304105MVZ
CSB-EP304105MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative glycosyltransferases (pimF), partial CSB-YP304106MVZ1
CSB-EP304106MVZ1
CSB-BP304106MVZ1
CSB-MP304106MVZ1
CSB-EP304106MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0142 protein Rv1375/MT1419 (Rv1375, MT1419) CSB-YP304107MVZ
CSB-EP304107MVZ
CSB-BP304107MVZ
CSB-MP304107MVZ
CSB-EP304107MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative CoA-transferase subunit alpha Rv3551/MT3655 (Rv3551, MT3655) CSB-YP304108MVZ
CSB-EP304108MVZ
CSB-BP304108MVZ
CSB-MP304108MVZ
CSB-EP304108MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-ketosteroid-9-alpha-hydroxylase oxygenase subunit (kshA) CSB-YP304109MVZ
CSB-EP304109MVZ
CSB-BP304109MVZ
CSB-MP304109MVZ
CSB-EP304109MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxin Rv2653c/MT2730 (Rv2653c, MT2730) CSB-YP304110MVZ
CSB-EP304110MVZ
CSB-BP304110MVZ
CSB-MP304110MVZ
CSB-EP304110MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ribonuclease VapC12 (vapC12) CSB-YP304111MVZ
CSB-EP304111MVZ
CSB-BP304111MVZ
CSB-MP304111MVZ
CSB-EP304111MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable sulfate transporter Rv1739c/MT1781 (Rv1739c, MT1781), partial CSB-YP304112MVZ
CSB-EP304112MVZ
CSB-BP304112MVZ
CSB-MP304112MVZ
CSB-EP304112MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-1-phosphate thymidylyltransferase (rmlA) CSB-YP304113MVZ
CSB-EP304113MVZ
CSB-BP304113MVZ
CSB-MP304113MVZ
CSB-EP304113MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv3778c/MT3887 (Rv3778c, MT3887) CSB-YP304114MVZ
CSB-EP304114MVZ
CSB-BP304114MVZ
CSB-MP304114MVZ
CSB-EP304114MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase (lst) CSB-YP304115NGG
CSB-EP304115NGG
CSB-BP304115NGG
CSB-MP304115NGG
CSB-EP304115NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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