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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Zea mays Unknown protein from spot 146 of 2D-PAGE of etiolated coleoptile CSB-YP305328ZAX
CSB-EP305328ZAX
CSB-BP305328ZAX
CSB-MP305328ZAX
CSB-EP305328ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 1131 of 2D-PAGE of etiolated coleoptile CSB-YP305329ZAX
CSB-EP305329ZAX
CSB-BP305329ZAX
CSB-MP305329ZAX
CSB-EP305329ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 159 of 2D-PAGE of etiolated coleoptile CSB-YP305330ZAX
CSB-EP305330ZAX
CSB-BP305330ZAX
CSB-MP305330ZAX
CSB-EP305330ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 207 of 2D-PAGE of etiolated coleoptile CSB-YP305331ZAX
CSB-EP305331ZAX
CSB-BP305331ZAX
CSB-MP305331ZAX
CSB-EP305331ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 245 of 2D-PAGE of etiolated coleoptile CSB-YP305332ZAX
CSB-EP305332ZAX
CSB-BP305332ZAX
CSB-MP305332ZAX
CSB-EP305332ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 406 of 2D-PAGE of etiolated coleoptile CSB-YP305333ZAX
CSB-EP305333ZAX
CSB-BP305333ZAX
CSB-MP305333ZAX
CSB-EP305333ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 502 of 2D-PAGE of etiolated coleoptile CSB-YP305334ZAX
CSB-EP305334ZAX
CSB-BP305334ZAX
CSB-MP305334ZAX
CSB-EP305334ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 984 of 2D-PAGE of etiolated coleoptile CSB-YP305335ZAX
CSB-EP305335ZAX
CSB-BP305335ZAX
CSB-MP305335ZAX
CSB-EP305335ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 75 of 2D-PAGE of etiolated coleoptile CSB-YP305336ZAX
CSB-EP305336ZAX
CSB-BP305336ZAX
CSB-MP305336ZAX
CSB-EP305336ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 415 of 2D-PAGE of etiolated coleoptile CSB-YP305337ZAX
CSB-EP305337ZAX
CSB-BP305337ZAX
CSB-MP305337ZAX
CSB-EP305337ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli FMN reductase (NADPH) (ssuE) CSB-YP305338ENV
CSB-EP305338ENV
CSB-MP305338ENV
CSB-EP305338ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Tetrahydromethanopterin S-methyltransferase subunit H (mtrH) CSB-YP305339MSN
CSB-EP305339MSN
CSB-BP305339MSN
CSB-MP305339MSN
CSB-EP305339MSN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Tetrahydromethanopterin S-methyltransferase subunit D (mtrD), partial CSB-YP305340MSN1
CSB-EP305340MSN1
CSB-BP305340MSN1
CSB-MP305340MSN1
CSB-EP305340MSN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Oxygen-evolving enhancer protein 2 CSB-YP305341EVD
CSB-EP305341EVD
CSB-BP305341EVD
CSB-MP305341EVD
CSB-EP305341EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Peroxisomal membrane protein PAS20 (PEX13), partial CSB-YP305342SVG
CSB-EP305342SVG
CSB-BP305342SVG
CSB-MP305342SVG
CSB-EP305342SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Potassium channel toxin alpha-KTx 8.4 CSB-YP305343LDS
CSB-EP305343LDS
CSB-BP305343LDS
CSB-MP305343LDS
CSB-EP305343LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blaberus craniifer Cuticle protein 2 CSB-YP305344BHA
CSB-EP305344BHA
CSB-BP305344BHA
CSB-MP305344BHA
CSB-EP305344BHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chelyosoma productum Gonadoliberin-2 CSB-YP305345CHY
CSB-EP305345CHY
CSB-BP305345CHY
CSB-MP305345CHY
CSB-EP305345CHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tenebrio molitor Larval cuticle protein A3A CSB-YP305346TBF
CSB-EP305346TBF
CSB-BP305346TBF
CSB-MP305346TBF
CSB-EP305346TBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carica papaya Latex serine proteinase inhibitor CSB-YP305347DQH
CSB-EP305347DQH
CSB-BP305347DQH
CSB-MP305347DQH
CSB-EP305347DQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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