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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Candida albicans Putative NADPH-dependent methylglyoxal reductase GRP2 (GRP2) CSB-YP307396CZD
CSB-EP307396CZD
CSB-BP307396CZD
CSB-MP307396CZD
CSB-EP307396CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Mitochondrial outer membrane protein porin (POR1) CSB-YP307397CZD
CSB-EP307397CZD
CSB-BP307397CZD
CSB-MP307397CZD
CSB-EP307397CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mastigocladus laminosus Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP307398MQJ1
CSB-EP307398MQJ1
CSB-BP307398MQJ1
CSB-MP307398MQJ1
CSB-EP307398MQJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bubble protein CSB-YP307399PES
CSB-EP307399PES
CSB-BP307399PES
CSB-MP307399PES
CSB-EP307399PES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eisenia hortensis Chymotrypsin inhibitor CSB-YP307400EBC
CSB-EP307400EBC
CSB-BP307400EBC
CSB-MP307400EBC
CSB-EP307400EBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viola arvensis Varv peptide E CSB-YP307401VCY
CSB-EP307401VCY
CSB-BP307401VCY
CSB-MP307401VCY
CSB-EP307401VCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litopenaeus vannamei Histone H3 CSB-YP307402LHD
CSB-EP307402LHD
CSB-BP307402LHD
CSB-MP307402LHD
CSB-EP307402LHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Immune-induced peptide 14 (IM14) CSB-YP307403DLU
CSB-EP307403DLU
CSB-BP307403DLU
CSB-MP307403DLU
CSB-EP307403DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria reidyi U9-ctenitoxin-Pr1a CSB-YP307404PHX
CSB-EP307404PHX
CSB-BP307404PHX
CSB-MP307404PHX
CSB-EP307404PHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naegleria fowleri Unknown protein NF019 from 2D-PAGE CSB-YP307405NAA
CSB-EP307405NAA
CSB-BP307405NAA
CSB-MP307405NAA
CSB-EP307405NAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria keyserlingi U2-ctenitoxin-Pk1a CSB-YP307406PHW
CSB-EP307406PHW
CSB-BP307406PHW
CSB-MP307406PHW
CSB-EP307406PHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria keyserlingi U6-ctenitoxin-Pk1a CSB-YP307407PHW
CSB-EP307407PHW
CSB-BP307407PHW
CSB-MP307407PHW
CSB-EP307407PHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhyparobia maderae Periviscerokinin-2 CSB-YP307408REJ
CSB-EP307408REJ
CSB-BP307408REJ
CSB-MP307408REJ
CSB-EP307408REJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blaptica dubia Periviscerokinin-3 CSB-YP307409BHE
CSB-EP307409BHE
CSB-BP307409BHE
CSB-MP307409BHE
CSB-EP307409BHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kalata-B4 CSB-YP307410OAZ
CSB-EP307410OAZ
CSB-BP307410OAZ
CSB-MP307410OAZ
CSB-EP307410OAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus eugenii Transcriptional regulator ATRY (ATRY) CSB-YP307411MPE
CSB-EP307411MPE
CSB-BP307411MPE
CSB-MP307411MPE
CSB-EP307411MPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyuranus microlepidotus Omwaprin-a CSB-YP307412OGE
CSB-EP307412OGE
CSB-BP307412OGE
CSB-MP307412OGE
CSB-EP307412OGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Castanopsis chinensis Thaumatin-like protein CSB-YP307413CEM
CSB-EP307413CEM
CSB-BP307413CEM
CSB-MP307413CEM
CSB-EP307413CEM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bactrocera dorsalis Actin, indirect flight muscle CSB-YP307414BDX
CSB-EP307414BDX
CSB-BP307414BDX
CSB-MP307414BDX
CSB-EP307414BDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus stolonifer Alpha- (1-6)-linked fucose-specific lectin CSB-YP307415RDF
CSB-EP307415RDF
CSB-BP307415RDF
CSB-MP307415RDF
CSB-EP307415RDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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