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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Antimicrobial peptide LCI CSB-YP306773BRI
CSB-EP306773BRI
CSB-BP306773BRI
CSB-MP306773BRI
CSB-EP306773BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allergen Asp fl 3 CSB-YP306774AVD
CSB-EP306774AVD
CSB-BP306774AVD
CSB-MP306774AVD
CSB-EP306774AVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea 30S ribosomal protein S9, chloroplastic (PRPS9) CSB-YP306775FKI
CSB-EP306775FKI
CSB-BP306775FKI
CSB-MP306775FKI
CSB-EP306775FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombina variegata Bombinin-H4 CSB-YP306776BIC
CSB-EP306776BIC
CSB-BP306776BIC
CSB-MP306776BIC
CSB-EP306776BIC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Hemocyanin subunit 6 CSB-YP306777HWO
CSB-EP306777HWO
CSB-BP306777HWO
CSB-MP306777HWO
CSB-EP306777HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carcinus maenas Hemocyanin subunit 4 CSB-YP306778CDS
CSB-EP306778CDS
CSB-BP306778CDS
CSB-MP306778CDS
CSB-EP306778CDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Unknown protein from spot 108 of 2D-PAGE of thylakoid CSB-YP306779EWE
CSB-EP306779EWE
CSB-BP306779EWE
CSB-MP306779EWE
CSB-EP306779EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Unknown protein from spot 119 of 2D-PAGE of thylakoid CSB-YP306780EWE
CSB-EP306780EWE
CSB-BP306780EWE
CSB-MP306780EWE
CSB-EP306780EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pagothenia borchgrevinki Hemoglobin subunit beta-0 (hbb0) CSB-YP306781EQQ
CSB-EP306781EQQ
CSB-BP306781EQQ
CSB-MP306781EQQ
CSB-EP306781EQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prunus armeniaca Non-specific lipid-transfer protein 2 CSB-YP306782EZH
CSB-EP306782EZH
CSB-BP306782EZH
CSB-MP306782EZH
CSB-EP306782EZH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Fructokinase (scrK) CSB-YP306783LNF
CSB-EP306783LNF
CSB-BP306783LNF
CSB-MP306783LNF
CSB-EP306783LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria aurea Aurein-2.2 CSB-YP306784LHE
CSB-EP306784LHE
CSB-BP306784LHE
CSB-MP306784LHE
CSB-EP306784LHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Momordica cochinchinensis Trypsin inhibitor 1 CSB-YP306785MHR
CSB-EP306785MHR
CSB-BP306785MHR
CSB-MP306785MHR
CSB-EP306785MHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pachycondyla goeldii Ponericin-G3 CSB-YP306786PAC
CSB-EP306786PAC
CSB-BP306786PAC
CSB-MP306786PAC
CSB-EP306786PAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pachycondyla goeldii Ponericin-W4 CSB-YP306787PAC
CSB-EP306787PAC
CSB-BP306787PAC
CSB-MP306787PAC
CSB-EP306787PAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 28 kDa cell wall protein CSB-YP306788NHE
CSB-EP306788NHE
CSB-BP306788NHE
CSB-MP306788NHE
CSB-EP306788NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 50 kDa cell wall protein CSB-YP306789NHE
CSB-EP306789NHE
CSB-BP306789NHE
CSB-MP306789NHE
CSB-EP306789NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja kaouthia Muscarinic toxin-like protein 1 CSB-YP306790NAF
CSB-EP306790NAF
CSB-BP306790NAF
CSB-MP306790NAF
CSB-EP306790NAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Alveolar macrophage chemotactic factor CSB-YP306791RB
CSB-EP306791RB
CSB-BP306791RB
CSB-MP306791RB
CSB-EP306791RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-N-arabinofuranosidase 1, partial CSB-YP306792SNW
CSB-EP306792SNW
CSB-BP306792SNW
CSB-MP306792SNW
CSB-EP306792SNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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