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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pinus monticola Antimicrobial peptide 1 CSB-YP307978PKL
CSB-EP307978PKL
CSB-BP307978PKL
CSB-MP307978PKL
CSB-EP307978PKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naegleria fowleri Unknown protein NF015 from 2D-PAGE CSB-YP307979NAA
CSB-EP307979NAA
CSB-BP307979NAA
CSB-MP307979NAA
CSB-EP307979NAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria keyserlingi U4-ctenitoxin-Pk1a CSB-YP307980PHW
CSB-EP307980PHW
CSB-BP307980PHW
CSB-MP307980PHW
CSB-EP307980PHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria reidyi U1-ctenitoxin-Pr1a CSB-YP307981PHX
CSB-EP307981PHX
CSB-BP307981PHX
CSB-MP307981PHX
CSB-EP307981PHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria reidyi U2-ctenitoxin-Pr1a CSB-YP307982PHX
CSB-EP307982PHX
CSB-BP307982PHX
CSB-MP307982PHX
CSB-EP307982PHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Dermaseptin-like peptide CSB-YP307983SXQ
CSB-EP307983SXQ
CSB-BP307983SXQ
CSB-MP307983SXQ
CSB-EP307983SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blaptica dubia Periviscerokinin-1 CSB-YP307984BHE
CSB-EP307984BHE
CSB-BP307984BHE
CSB-MP307984BHE
CSB-EP307984BHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gromphadorina portentosa Periviscerokinin-2 CSB-YP307985GDM
CSB-EP307985GDM
CSB-BP307985GDM
CSB-MP307985GDM
CSB-EP307985GDM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gromphadorina portentosa Periviscerokinin-3 CSB-YP307986GDM
CSB-EP307986GDM
CSB-BP307986GDM
CSB-MP307986GDM
CSB-EP307986GDM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ficus pumila var. awkeotsang Pectinesterase/pectinesterase inhibitor CSB-YP307987FBI
CSB-EP307987FBI
CSB-BP307987FBI
CSB-MP307987FBI
CSB-EP307987FBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vigna unguiculata subsp. sesquipedalis Lectin 31 kDa subunit CSB-YP307988VCW
CSB-EP307988VCW
CSB-BP307988VCW
CSB-MP307988VCW
CSB-EP307988VCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Lysozyme D (LysD) CSB-YP307989DLU
CSB-EP307989DLU
CSB-BP307989DLU
CSB-MP307989DLU
CSB-EP307989DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ginglymostoma cirratum Pancreatic IgW, short secretory form CSB-YP307990GCE
CSB-EP307990GCE
CSB-BP307990GCE
CSB-MP307990GCE
CSB-EP307990GCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 12.5 CSB-YP307991LHY
CSB-EP307991LHY
CSB-BP307991LHY
CSB-MP307991LHY
CSB-EP307991LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 18.6, isoform A CSB-YP307992LHY
CSB-EP307992LHY
CSB-BP307992LHY
CSB-MP307992LHY
CSB-EP307992LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria reidyi U10-ctenitoxin-Pr1a CSB-YP307993PHX
CSB-EP307993PHX
CSB-BP307993PHX
CSB-MP307993PHX
CSB-EP307993PHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria nigriventer U11-ctenitoxin-Pn1b CSB-YP307994EUV
CSB-EP307994EUV
CSB-BP307994EUV
CSB-MP307994EUV
CSB-EP307994EUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria nigriventer U23-ctenitoxin-Pn1a CSB-YP307995EUV
CSB-EP307995EUV
CSB-BP307995EUV
CSB-MP307995EUV
CSB-EP307995EUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria reidyi U7-ctenitoxin-Pr1a CSB-YP307996PHX
CSB-EP307996PHX
CSB-BP307996PHX
CSB-MP307996PHX
CSB-EP307996PHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina Lebecetin subunit alpha CSB-YP307997MPJ
CSB-EP307997MPJ
CSB-BP307997MPJ
CSB-MP307997MPJ
CSB-EP307997MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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