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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human herpesvirus 2 Trans-acting transcriptional protein ICP4 (ICP4), partial CSB-YP308583HJX
CSB-EP308583HJX
CSB-BP308583HJX
CSB-MP308583HJX
CSB-EP308583HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Opsin, blue-sensitive (BLOP), partial CSB-YP308584DNK1
CSB-EP308584DNK1
CSB-BP308584DNK1
CSB-MP308584DNK1
CSB-EP308584DNK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0392 protein C27A7.2 (C27A7.2) CSB-YP308585CXY
CSB-EP308585CXY
CSB-BP308585CXY
CSB-MP308585CXY
CSB-EP308585CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable histone-binding protein rba-1 (rba-1) CSB-YP308586CXY
CSB-EP308586CXY
CSB-BP308586CXY
CSB-MP308586CXY
CSB-EP308586CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant T-box protein 12 (mab-9) CSB-YP308587CXY
CSB-EP308587CXY
CSB-BP308587CXY
CSB-MP308587CXY
CSB-EP308587CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serpentine receptor class delta-25 (srd-25), partial CSB-YP308589CXY1
CSB-EP308589CXY1
CSB-BP308589CXY1
CSB-MP308589CXY1
CSB-EP308589CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable glutathione S-transferase 8 (gst-8) CSB-YP308590CXY
CSB-EP308590CXY
CSB-BP308590CXY
CSB-MP308590CXY
CSB-EP308590CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative protein phosphatase tag-83 (tag-83) CSB-YP308591CXY
CSB-EP308591CXY
CSB-BP308591CXY
CSB-MP308591CXY
CSB-EP308591CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spindle-defective protein 5 (spd-5), partial CSB-YP308592CXY
CSB-EP308592CXY
CSB-BP308592CXY
CSB-MP308592CXY
CSB-EP308592CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative bolA-like protein K11H12.1 (K11H12.1) CSB-YP308593CXY
CSB-EP308593CXY
CSB-BP308593CXY
CSB-MP308593CXY
CSB-EP308593CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dimethyladenosine transferase 1, mitochondrial (T03F1.7) CSB-YP308594CXY
CSB-EP308594CXY
CSB-BP308594CXY
CSB-MP308594CXY
CSB-EP308594CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Warthog protein 6 (wrt-6), partial CSB-YP308595CXY
CSB-EP308595CXY
CSB-BP308595CXY
CSB-MP308595CXY
CSB-EP308595CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila nebulosa Period circadian protein (per) CSB-YP308596DGM
CSB-EP308596DGM
CSB-BP308596DGM
CSB-MP308596DGM
CSB-EP308596DGM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lytechinus pictus Mitotic apparatus protein p62 CSB-YP308597LRW
CSB-EP308597LRW
CSB-BP308597LRW
CSB-MP308597LRW
CSB-EP308597LRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymnaea stagnalis Molluscan insulin-related peptide 7 CSB-YP308598LRQ
CSB-EP308598LRQ
CSB-BP308598LRQ
CSB-MP308598LRQ
CSB-EP308598LRQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratitis capitata Heat shock protein 70, partial CSB-YP308599DRF
CSB-EP308599DRF
CSB-BP308599DRF
CSB-MP308599DRF
CSB-EP308599DRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative mitochondrial inner membrane protein (CG6455), partial CSB-YP308600DLU1
CSB-EP308600DLU1
CSB-BP308600DLU1
CSB-MP308600DLU1
CSB-EP308600DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus galloprovincialis Tropomyosin CSB-YP308601MWV
CSB-EP308601MWV
CSB-BP308601MWV
CSB-MP308601MWV
CSB-EP308601MWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Larval cuticle protein 8 (Lcp65Ag1) CSB-YP308602DLU
CSB-EP308602DLU
CSB-BP308602DLU
CSB-MP308602DLU
CSB-EP308602DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agropyron cristatum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP308603AFH
CSB-EP308603AFH
CSB-BP308603AFH
CSB-MP308603AFH
CSB-EP308603AFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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