Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Chlamydomonas reinhardtii Photosystem II reaction center protein M (psbM), partial CSB-YP308604DSJ
CSB-EP308604DSJ
CSB-BP308604DSJ
CSB-MP308604DSJ
CSB-EP308604DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP308605PTC
CSB-EP308605PTC
CSB-BP308605PTC
CSB-MP308605PTC
CSB-EP308605PTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00920 (AtMg00920) CSB-YP308606DOA
CSB-EP308606DOA
CSB-BP308606DOA
CSB-MP308606DOA
CSB-EP308606DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg01040 (AtMg01040) CSB-YP308607DOA
CSB-EP308607DOA
CSB-BP308607DOA
CSB-MP308607DOA
CSB-EP308607DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg01220 (AtMg01220) CSB-YP308608DOA
CSB-EP308608DOA
CSB-BP308608DOA
CSB-MP308608DOA
CSB-EP308608DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg01400 (AtMg01400) CSB-YP308609DOA
CSB-EP308609DOA
CSB-BP308609DOA
CSB-MP308609DOA
CSB-EP308609DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aegilops speltoides DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP308610AXS
CSB-EP308610AXS
CSB-BP308610AXS
CSB-MP308610AXS
CSB-EP308610AXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcium-transporting ATPase 1, endoplasmic reticulum-type (ECA1), partial CSB-YP308611DOA
CSB-EP308611DOA
CSB-BP308611DOA
CSB-MP308611DOA
CSB-EP308611DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Monodehydroascorbate reductase, chloroplastic (At1g63940) CSB-YP308612DOA
CSB-EP308612DOA
CSB-BP308612DOA
CSB-MP308612DOA
CSB-EP308612DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Arginine/serine-rich-splicing factor RSP31 (RSP31) CSB-YP308613DOA
CSB-EP308613DOA
CSB-BP308613DOA
CSB-MP308613DOA
CSB-EP308613DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable polygalacturonase non-catalytic subunit JP650 (JP650), partial CSB-YP308615DOA
CSB-EP308615DOA
CSB-BP308615DOA
CSB-MP308615DOA
CSB-EP308615DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Germin-like protein subfamily 1 member 13 (GLP6) CSB-YP308616DOA
CSB-EP308616DOA
CSB-BP308616DOA
CSB-MP308616DOA
CSB-EP308616DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Regulatory protein NPR1 (NPR1), partial CSB-YP308617DOA
CSB-EP308617DOA
CSB-BP308617DOA
CSB-MP308617DOA
CSB-EP308617DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RMA2 (RMA2), partial CSB-YP308618DOA1
CSB-EP308618DOA1
CSB-BP308618DOA1
CSB-MP308618DOA1
CSB-EP308618DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chromatin structure-remodeling complex protein BSH (BSH) CSB-YP308619DOA
CSB-EP308619DOA
CSB-BP308619DOA
CSB-MP308619DOA
CSB-EP308619DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum 14-3-3 protein 6 (TFT6) CSB-YP308621SZY
CSB-EP308621SZY
CSB-BP308621SZY
CSB-MP308621SZY
CSB-EP308621SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Polygalacturonase non-catalytic subunit AroGP2 (GP2), partial CSB-YP308622SZY
CSB-EP308622SZY
CSB-BP308622SZY
CSB-MP308622SZY
CSB-EP308622SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Linum usitatissimum Aliphatic (R)-hydroxynitrile lyase CSB-YP308623LGG
CSB-EP308623LGG
CSB-BP308623LGG
CSB-MP308623LGG
CSB-EP308623LGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (METE), partial CSB-YP308624MRC
CSB-EP308624MRC
CSB-BP308624MRC
CSB-MP308624MRC
CSB-EP308624MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00240 (AtMg00240) CSB-YP308625DOA
CSB-EP308625DOA
CSB-BP308625DOA
CSB-MP308625DOA
CSB-EP308625DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>