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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00200 (AtMg00200) CSB-YP309011DOA
CSB-EP309011DOA
CSB-BP309011DOA
CSB-MP309011DOA
CSB-EP309011DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00320 (AtMg00320) CSB-YP309012DOA
CSB-EP309012DOA
CSB-BP309012DOA
CSB-MP309012DOA
CSB-EP309012DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00490 (AtMg00490) CSB-YP309013DOA
CSB-EP309013DOA
CSB-BP309013DOA
CSB-MP309013DOA
CSB-EP309013DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00610 (AtMg00610), partial CSB-YP309014DOA1
CSB-EP309014DOA1
CSB-BP309014DOA1
CSB-MP309014DOA1
CSB-EP309014DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Uracil phosphoribosyltransferase (UPP) CSB-YP309015NHE
CSB-EP309015NHE
CSB-BP309015NHE
CSB-MP309015NHE
CSB-EP309015NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrus pyrifolia Ribonuclease S-5 CSB-YP309016FIB
CSB-EP309016FIB
CSB-BP309016FIB
CSB-MP309016FIB
CSB-EP309016FIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorghum bicolor Phytochrome B (PHYB), partial CSB-YP309017FJS
CSB-EP309017FJS
CSB-BP309017FJS
CSB-MP309017FJS
CSB-EP309017FJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Styphnolobium japonicum Seed lectin CSB-YP309018SRB
CSB-EP309018SRB
CSB-BP309018SRB
CSB-MP309018SRB
CSB-EP309018SRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Guanine nucleotide-binding protein alpha-1 subunit (GPA1) CSB-YP309019FIG
CSB-EP309019FIG
CSB-BP309019FIG
CSB-MP309019FIG
CSB-EP309019FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Actin-65 CSB-YP309020FIG
CSB-EP309020FIG
CSB-BP309020FIG
CSB-MP309020FIG
CSB-EP309020FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Lon protease homolog, mitochondrial (LON2), partial CSB-YP309021ZAX
CSB-EP309021ZAX
CSB-BP309021ZAX
CSB-MP309021ZAX
CSB-EP309021ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative actin-9 (ACT9) CSB-YP309022DOA
CSB-EP309022DOA
CSB-BP309022DOA
CSB-MP309022DOA
CSB-EP309022DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Phosphoglucomutase, cytoplasmic 1, partial CSB-YP309023ZAX
CSB-EP309023ZAX
CSB-BP309023ZAX
CSB-MP309023ZAX
CSB-EP309023ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 1-aminocyclopropane-1-carboxylate oxidase homolog 6 (At1g04350) CSB-YP309024DOA
CSB-EP309024DOA
CSB-BP309024DOA
CSB-MP309024DOA
CSB-EP309024DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorghum bicolor Obtusifoliol 14-alpha demethylase (CYP51), partial CSB-YP309025FJS1
CSB-EP309025FJS1
CSB-BP309025FJS1
CSB-MP309025FJS1
CSB-EP309025FJS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP309026WBI
CSB-EP309026WBI
CSB-BP309026WBI
CSB-MP309026WBI
CSB-EP309026WBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cynodon dactylon Polcalcin Cyn d 7 CSB-YP309027EQB
CSB-EP309027EQB
CSB-BP309027EQB
CSB-MP309027EQB
CSB-EP309027EQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Cis,cis-muconate transport protein (mucK), partial CSB-YP309028AWW
CSB-EP309028AWW
CSB-BP309028AWW
CSB-MP309028AWW
CSB-EP309028AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae 10 kDa chaperonin (groS) CSB-YP309029AXG
CSB-EP309029AXG
CSB-BP309029AXG
CSB-MP309029AXG
CSB-EP309029AXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcaligenes sp. Cation efflux system protein CzcD (czcD), partial CSB-YP309030AGQ1
CSB-EP309030AGQ1
CSB-BP309030AGQ1
CSB-MP309030AGQ1
CSB-EP309030AGQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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