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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pinus strobus Putative fructose-bisphosphate aldolase, chloroplastic CSB-YP307536EVX
CSB-EP307536EVX
CSB-BP307536EVX
CSB-MP307536EVX
CSB-EP307536EVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus strobus Putative NADH dehydrogenase subunit PS9 CSB-YP307537EVX
CSB-EP307537EVX
CSB-BP307537EVX
CSB-MP307537EVX
CSB-EP307537EVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus strobus Putative 2-Cys peroxiredoxin BAS1 CSB-YP307538EVX
CSB-EP307538EVX
CSB-BP307538EVX
CSB-MP307538EVX
CSB-EP307538EVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus strobus Putative LRR disease resistance protein/transmembrane receptor kinase PS16 CSB-YP307539EVX
CSB-EP307539EVX
CSB-BP307539EVX
CSB-MP307539EVX
CSB-EP307539EVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus strobus Putative LRR disease resistance protein/transmembrane receptor kinase PS19 CSB-YP307540EVX
CSB-EP307540EVX
CSB-BP307540EVX
CSB-MP307540EVX
CSB-EP307540EVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photoactivated adenylate cyclase subunit alpha-like protein 1224-5/1F (pacA), partial CSB-YP307541EHU
CSB-EP307541EHU
CSB-BP307541EHU
CSB-MP307541EHU
CSB-EP307541EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photoactivated adenylate cyclase subunit beta-like protein FB (pacB), partial CSB-YP307542EHU
CSB-EP307542EHU
CSB-BP307542EHU
CSB-MP307542EHU
CSB-EP307542EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photoactivated adenylate cyclase subunit beta-like protein ST- (pacB), partial CSB-YP307543EHU
CSB-EP307543EHU
CSB-BP307543EHU
CSB-MP307543EHU
CSB-EP307543EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararacussu 1095.6 Da venom vasodilator peptide CSB-YP307544BUX
CSB-EP307544BUX
CSB-BP307544BUX
CSB-MP307544BUX
CSB-EP307544BUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Argiope aurantia Astacin-like peptidase p18 CSB-YP307545ANV
CSB-EP307545ANV
CSB-BP307545ANV
CSB-MP307545ANV
CSB-EP307545ANV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig kappa chain V region Mem5 CSB-YP307546MO
CSB-EP307546MO
CSB-BP307546MO
CSB-MP307546MO
CSB-EP307546MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aloe vera Peroxidase B CSB-YP307547AHS
CSB-EP307547AHS
CSB-BP307547AHS
CSB-MP307547AHS
CSB-EP307547AHS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyopes lineatus Omega-oxotoxin-Ol1b CSB-YP307548ODB
CSB-EP307548ODB
CSB-BP307548ODB
CSB-MP307548ODB
CSB-EP307548ODB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Jellein-3 CSB-YP307549DNK
CSB-EP307549DNK
CSB-BP307549DNK
CSB-MP307549DNK
CSB-EP307549DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tricholoma giganteum Angiotensin-1-converting enzyme inhibitory peptide CSB-YP307550TIC
CSB-EP307550TIC
CSB-BP307550TIC
CSB-MP307550TIC
CSB-EP307550TIC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 (rpmG) CSB-YP307551PUO
CSB-EP307551PUO
CSB-BP307551PUO
CSB-MP307551PUO
CSB-EP307551PUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aegilops tauschii subsp. strangulata Granule-bound starch synthase 1 CSB-YP307552ADK
CSB-EP307552ADK
CSB-BP307552ADK
CSB-MP307552ADK
CSB-EP307552ADK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Insulin receptor substrate 1-B (irs1-b), partial CSB-YP307553XBE
CSB-EP307553XBE
CSB-BP307553XBE
CSB-MP307553XBE
CSB-EP307553XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zhaoermia mangshanensis Phospholipase A2 homolog zhaoermiatoxin CSB-YP307554ZAH
CSB-EP307554ZAH
CSB-BP307554ZAH
CSB-MP307554ZAH
CSB-EP307554ZAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mirabilis jalapa Trypsin inhibitor 1 CSB-YP307555MGZ
CSB-EP307555MGZ
CSB-BP307555MGZ
CSB-MP307555MGZ
CSB-EP307555MGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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