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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR426C (YDR426C) CSB-YP308949SVG
CSB-EP308949SVG
CSB-BP308949SVG
CSB-MP308949SVG
CSB-EP308949SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR526C (YDR526C), partial CSB-YP308950SVG1
CSB-EP308950SVG1
CSB-BP308950SVG1
CSB-MP308950SVG1
CSB-EP308950SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative RNA polymerase II transcriptional coactivator (SPAC16A10.02) CSB-YP308951SXV
CSB-EP308951SXV
CSB-BP308951SXV
CSB-MP308951SXV
CSB-EP308951SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Translation machinery-associated protein 20 (tma20) CSB-YP308952SXV
CSB-EP308952SXV
CSB-BP308952SXV
CSB-MP308952SXV
CSB-EP308952SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Protein msp1, mitochondrial (msp1), partial CSB-YP308953SXV
CSB-EP308953SXV
CSB-BP308953SXV
CSB-MP308953SXV
CSB-EP308953SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Columba livia Nucleoside diphosphate kinase, mitochondrial (NME4) CSB-YP308954DVK
CSB-EP308954DVK
CSB-BP308954DVK
CSB-MP308954DVK
CSB-EP308954DVK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Red-sensitive opsin, partial CSB-YP308955OFH1
CSB-EP308955OFH1
CSB-BP308955OFH1
CSB-MP308955OFH1
CSB-EP308955OFH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 2 Maturation protein (PIVA2) CSB-YP308956CCN
CSB-EP308956CCN
CSB-BP308956CCN
CSB-MP308956CCN
CSB-EP308956CCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gibbon hepatitis B virus subtype ayw3q Protein P (P), partial CSB-YP308958GCB
CSB-EP308958GCB
CSB-BP308958GCB
CSB-MP308958GCB
CSB-EP308958GCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Intermediate capsid protein VP6 CSB-YP308959RGH
CSB-EP308959RGH
CSB-BP308959RGH
CSB-MP308959RGH
CSB-EP308959RGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus D serotype 9 E4-ORF1 (E4) CSB-YP308960HIP
CSB-EP308960HIP
CSB-BP308960HIP
CSB-MP308960HIP
CSB-EP308960HIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sweet potato mild mottle virus Genome polyprotein, partial CSB-YP308961SRS
CSB-EP308961SRS
CSB-BP308961SRS
CSB-MP308961SRS
CSB-EP308961SRS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tegument protein UL14 homolog (UL14) CSB-YP308962HJX
CSB-EP308962HJX
CSB-BP308962HJX
CSB-MP308962HJX
CSB-EP308962HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Protein UL21 (UL21), partial CSB-YP308963HJX
CSB-EP308963HJX
CSB-BP308963HJX
CSB-MP308963HJX
CSB-EP308963HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 DNA polymerase catalytic subunit (UL30), partial CSB-YP308964HJX
CSB-EP308964HJX
CSB-BP308964HJX
CSB-MP308964HJX
CSB-EP308964HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Capsid assembly protein UL37 (UL37), partial CSB-YP308965HJX
CSB-EP308965HJX
CSB-BP308965HJX
CSB-MP308965HJX
CSB-EP308965HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tegument protein UL47 (UL47), partial CSB-YP308966HJX
CSB-EP308966HJX
CSB-BP308966HJX
CSB-MP308966HJX
CSB-EP308966HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Protein US8.5 (US8.5) CSB-YP308967HJX
CSB-EP308967HJX
CSB-BP308967HJX
CSB-MP308967HJX
CSB-EP308967HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Odontoglossum ringspot virus Replicase large subunit, partial CSB-YP308968OAN
CSB-EP308968OAN
CSB-BP308968OAN
CSB-MP308968OAN
CSB-EP308968OAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco streak virus Replication protein 1a, partial CSB-YP308969TFN
CSB-EP308969TFN
CSB-BP308969TFN
CSB-MP308969TFN
CSB-EP308969TFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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