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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster Vanin-like protein 3 (CG32750), partial CSB-YP307938DLU
CSB-EP307938DLU
CSB-BP307938DLU
CSB-MP307938DLU
CSB-EP307938DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrothele gigas Mu-hexatoxin-Mg1a CSB-YP307939MAM
CSB-EP307939MAM
CSB-BP307939MAM
CSB-MP307939MAM
CSB-EP307939MAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Allergen Api m 6.03 / Api m 6.04 CSB-YP307940DNK
CSB-MP307940DNK
CSB-EP307940DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sepia officinalis Neuropeptide GWa CSB-YP307941SYJ
CSB-EP307941SYJ
CSB-BP307941SYJ
CSB-MP307941SYJ
CSB-EP307941SYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aralia elata Aralin B chain CSB-YP307942ANG
CSB-EP307942ANG
CSB-BP307942ANG
CSB-MP307942ANG
CSB-EP307942ANG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phyllomedusa sauvagei Proteinase inhibitor PSKP-2 CSB-YP307943EVA
CSB-EP307943EVA
CSB-BP307943EVA
CSB-MP307943EVA
CSB-EP307943EVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orconectes limosus Pigment-dispersing hormone C CSB-YP307944OBZ
CSB-EP307944OBZ
CSB-BP307944OBZ
CSB-MP307944OBZ
CSB-EP307944OBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eucommia ulmoides Antifungal peptide 2 CSB-YP307945EHN
CSB-EP307945EHN
CSB-BP307945EHN
CSB-MP307945EHN
CSB-EP307945EHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naegleria fowleri Unknown protein NF045 from 2D-PAGE CSB-YP307946NAA
CSB-EP307946NAA
CSB-BP307946NAA
CSB-MP307946NAA
CSB-EP307946NAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Boophilus microplus Kunitz-type serine protease inhibitor D CSB-YP307947BTV
CSB-EP307947BTV
CSB-BP307947BTV
CSB-MP307947BTV
CSB-EP307947BTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gobionotothen gibberifrons Hemoglobin subunit alpha-1 (hba1) CSB-YP307948GGX
CSB-EP307948GGX
CSB-BP307948GGX
CSB-MP307948GGX
CSB-EP307948GGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 78 kDa glucose-regulated protein homolog (bipA), partial CSB-YP307949DOZ
CSB-EP307949DOZ
CSB-BP307949DOZ
CSB-MP307949DOZ
CSB-EP307949DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupiennius salei M-ctenitoxin-Cs1a CSB-YP307950CUR
CSB-EP307950CUR
CSB-BP307950CUR
CSB-MP307950CUR
CSB-EP307950CUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orconectes limosus Molt-inhibiting hormone CSB-YP307951OBZ
CSB-EP307951OBZ
CSB-BP307951OBZ
CSB-MP307951OBZ
CSB-EP307951OBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Toxin Lqh4 CSB-YP307952LDS
CSB-EP307952LDS
CSB-BP307952LDS
CSB-MP307952LDS
CSB-EP307952LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Profilin LP04 (OsI_020954) CSB-YP307953OFF
CSB-EP307953OFF
CSB-BP307953OFF
CSB-MP307953OFF
CSB-EP307953OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Catalase-1 CSB-YP307954CNM
CSB-EP307954CNM
CSB-BP307954CNM
CSB-MP307954CNM
CSB-EP307954CNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant High plains virus Capsid protein CSB-YP307955HHD
CSB-EP307955HHD
CSB-BP307955HHD
CSB-MP307955HHD
CSB-EP307955HHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pomacea flagellata Agglutinin-1 CSB-YP307956PPH
CSB-EP307956PPH
CSB-BP307956PPH
CSB-MP307956PPH
CSB-EP307956PPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana esculenta Orexigenic neuropeptide 26RFa CSB-YP307957RAW
CSB-EP307957RAW
CSB-BP307957RAW
CSB-MP307957RAW
CSB-EP307957RAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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