Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Uridine nucleosidase (URH1) CSB-YP312897SVG
CSB-EP312897SVG
CSB-BP312897SVG
CSB-MP312897SVG
CSB-EP312897SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Large-conductance mechanosensitive channel (mscL), partial CSB-YP312898LAU1
CSB-EP312898LAU1
CSB-BP312898LAU1
CSB-MP312898LAU1
CSB-EP312898LAU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP312899LAU
CSB-EP312899LAU
CSB-BP312899LAU
CSB-MP312899LAU
CSB-EP312899LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Transcriptional repressor NrdR (nrdR) CSB-YP312900LAU
CSB-EP312900LAU
CSB-BP312900LAU
CSB-MP312900LAU
CSB-EP312900LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae CUE domain-containing protein CUE4 (CUE4) CSB-YP312901SVG
CSB-EP312901SVG
CSB-BP312901SVG
CSB-MP312901SVG
CSB-EP312901SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thr operon leader peptide (thrL) CSB-YP312902SYN
CSB-EP312902SYN
CSB-BP312902SYN
CSB-MP312902SYN
CSB-EP312902SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosome assembly protein RRB1 (RRB1), partial CSB-YP312903SVG
CSB-EP312903SVG
CSB-BP312903SVG
CSB-MP312903SVG
CSB-EP312903SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sister chromatid cohesion protein PDS5 (PDS5), partial CSB-YP312904SVG
CSB-EP312904SVG
CSB-BP312904SVG
CSB-MP312904SVG
CSB-EP312904SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YMR086W (YMR086W), partial CSB-YP312905SVG
CSB-EP312905SVG
CSB-BP312905SVG
CSB-MP312905SVG
CSB-EP312905SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Phosphoglycerate kinase (pgk) CSB-YP312906LAU
CSB-EP312906LAU
CSB-BP312906LAU
CSB-MP312906LAU
CSB-EP312906LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP312907LAU
CSB-EP312907LAU
CSB-BP312907LAU
CSB-MP312907LAU
CSB-EP312907LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Phosphopantetheine adenylyltransferase (coaD) CSB-YP312908LAU
CSB-EP312908LAU
CSB-BP312908LAU
CSB-MP312908LAU
CSB-EP312908LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Putative phosphotransferase LGAS_1123 (LGAS_1123) CSB-YP312909LAU
CSB-EP312909LAU
CSB-BP312909LAU
CSB-MP312909LAU
CSB-EP312909LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial intermembrane space cysteine motif-containing protein MIC14 (MIC14) CSB-YP312910SVG
CSB-EP312910SVG
CSB-BP312910SVG
CSB-MP312910SVG
CSB-EP312910SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum Uncharacterized protein CA_C3711 (CA_C3711) CSB-YP312911DUB
CSB-EP312911DUB
CSB-BP312911DUB
CSB-MP312911DUB
CSB-EP312911DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae TPR repeat-containing protein YMR018W (YMR018W), partial CSB-YP312912SVG
CSB-EP312912SVG
CSB-BP312912SVG
CSB-MP312912SVG
CSB-EP312912SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Stationary phase protein 3 (SPG3), partial CSB-YP312913SVG1
CSB-EP312913SVG1
CSB-BP312913SVG1
CSB-MP312913SVG1
CSB-EP312913SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Homoserine kinase (thrB) CSB-YP312914LAU
CSB-EP312914LAU
CSB-BP312914LAU
CSB-MP312914LAU
CSB-EP312914LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Formate--tetrahydrofolate ligase (fhs), partial CSB-YP312915LAU
CSB-EP312915LAU
CSB-BP312915LAU
CSB-MP312915LAU
CSB-EP312915LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri CCA-adding enzyme (cca) CSB-YP312916LAU
CSB-EP312916LAU
CSB-BP312916LAU
CSB-MP312916LAU
CSB-EP312916LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>