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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human coronavirus OC43 Replicase polyprotein 1a (1a), partial CSB-YP313715HIY
CSB-EP313715HIY
CSB-BP313715HIY
CSB-MP313715HIY
CSB-EP313715HIY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine transmissible gastroenteritis coronavirus Replicase polyprotein 1a (1a), partial CSB-YP313716PQH
CSB-EP313716PQH
CSB-BP313716PQH
CSB-MP313716PQH
CSB-EP313716PQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Replicase polyprotein 1a (1a), partial CSB-YP313717ARW
CSB-EP313717ARW
CSB-BP313717ARW
CSB-MP313717ARW
CSB-EP313717ARW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus 279/2005 Replicase polyprotein 1ab (rep), partial CSB-YP313718BFB
CSB-EP313718BFB
CSB-BP313718BFB
CSB-MP313718BFB
CSB-EP313718BFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus 133/2005 Replicase polyprotein 1ab (rep), partial CSB-YP313719BFA
CSB-EP313719BFA
CSB-BP313719BFA
CSB-MP313719BFA
CSB-EP313719BFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU5 Replicase polyprotein 1ab (rep), partial CSB-YP313720BOU
CSB-EP313720BOU
CSB-BP313720BOU
CSB-MP313720BOU
CSB-EP313720BOU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Replicase polyprotein 1ab (rep), partial CSB-YP313721BJK
CSB-EP313721BJK
CSB-BP313721BJK
CSB-MP313721BJK
CSB-EP313721BJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human coronavirus 229E Replicase polyprotein 1ab (rep), partial CSB-YP313722HIT
CSB-EP313722HIT
CSB-BP313722HIT
CSB-MP313722HIT
CSB-EP313722HIT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human coronavirus HKU1 Replicase polyprotein 1ab (rep), partial CSB-YP313723HIW
CSB-EP313723HIW
CSB-BP313723HIW
CSB-MP313723HIW
CSB-EP313723HIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Replicase polyprotein 1ab (rep), partial CSB-YP313724MJV
CSB-EP313724MJV
CSB-BP313724MJV
CSB-MP313724MJV
CSB-EP313724MJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Replicase polyprotein 1ab (rep), partial CSB-YP313725ARK
CSB-EP313725ARK
CSB-BP313725ARK
CSB-MP313725ARK
CSB-EP313725ARK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine transmissible gastroenteritis coronavirus Replicase polyprotein 1ab (rep), partial CSB-YP313726PQH
CSB-EP313726PQH
CSB-BP313726PQH
CSB-MP313726PQH
CSB-EP313726PQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid protein VP4, partial CSB-YP313727RGZ
CSB-EP313727RGZ
CSB-BP313727RGZ
CSB-MP313727RGZ
CSB-EP313727RGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Glycoprotein N (gN), partial CSB-YP313728EEZ1
CSB-EP313728EEZ1
CSB-BP313728EEZ1
CSB-MP313728EEZ1
CSB-EP313728EEZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Glycoprotein 42 (BZLF2), partial CSB-YP313729EFC
CSB-BP313729EFC
CSB-MP313729EFC
CSB-EP313729EFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Serine/threonine-protein kinase BGLF4 (BGLF4) CSB-YP313730EEZ
CSB-EP313730EEZ
CSB-BP313730EEZ
CSB-MP313730EEZ
CSB-EP313730EEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Virion packaging protein BVRF1 (BVRF1), partial CSB-YP313731EEZ
CSB-EP313731EEZ
CSB-BP313731EEZ
CSB-MP313731EEZ
CSB-EP313731EEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 6 CSB-YP313732ROC
CSB-EP313732ROC
CSB-BP313732ROC
CSB-MP313732ROC
CSB-EP313732ROC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Transcriptional activator BRRF1 (BRRF1) CSB-YP313733EEZ
CSB-EP313733EEZ
CSB-BP313733EEZ
CSB-MP313733EEZ
CSB-EP313733EEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Uncharacterized protein LF3 (LF3), partial CSB-YP313734EFA
CSB-EP313734EFA
CSB-BP313734EFA
CSB-MP313734EFA
CSB-EP313734EFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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