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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus brevis Protein translocase subunit SecA (secA), partial CSB-YP312857LAL
CSB-EP312857LAL
CSB-BP312857LAL
CSB-MP312857LAL
CSB-EP312857LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis 50S ribosomal protein L33 1 (rpmG1) CSB-YP312858LAL
CSB-EP312858LAL
CSB-BP312858LAL
CSB-MP312858LAL
CSB-EP312858LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis 33 kDa chaperonin (hslO) CSB-YP312859LAL
CSB-EP312859LAL
CSB-BP312859LAL
CSB-MP312859LAL
CSB-EP312859LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Protease HtpX homolog (htpX), partial CSB-YP312860LAL1
CSB-EP312860LAL1
CSB-BP312860LAL1
CSB-MP312860LAL1
CSB-EP312860LAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis 30S ribosomal protein S14 (rpsN) CSB-YP312861LAL
CSB-EP312861LAL
CSB-BP312861LAL
CSB-MP312861LAL
CSB-EP312861LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis 50S ribosomal protein L9 (rplI) CSB-YP312862LAL
CSB-EP312862LAL
CSB-BP312862LAL
CSB-MP312862LAL
CSB-EP312862LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP312863LAL
CSB-EP312863LAL
CSB-BP312863LAL
CSB-MP312863LAL
CSB-EP312863LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides UPF0397 protein LEUM_1974 (LEUM_1974), partial CSB-YP312864LFG1
CSB-EP312864LFG1
CSB-BP312864LFG1
CSB-MP312864LFG1
CSB-EP312864LFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides NAD (P)H-hydrate epimerase CSB-YP312865LFG
CSB-EP312865LFG
CSB-BP312865LFG
CSB-MP312865LFG
CSB-EP312865LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Adapter protein mecA (mecA) CSB-YP312866LFG
CSB-EP312866LFG
CSB-BP312866LFG
CSB-MP312866LFG
CSB-EP312866LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Phosphoenolpyruvate carboxylase (ppcA), partial CSB-YP312867LFG
CSB-EP312867LFG
CSB-BP312867LFG
CSB-MP312867LFG
CSB-EP312867LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 30S ribosomal protein S20 (rpsT) CSB-YP312868LFG
CSB-EP312868LFG
CSB-BP312868LFG
CSB-MP312868LFG
CSB-EP312868LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP312869LFG
CSB-EP312869LFG
CSB-BP312869LFG
CSB-MP312869LFG
CSB-EP312869LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Probable GTP-binding protein EngB (engB) CSB-YP312870LFG
CSB-EP312870LFG
CSB-BP312870LFG
CSB-MP312870LFG
CSB-EP312870LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 30S ribosomal protein S16 (rpsP) CSB-YP312871LFG
CSB-EP312871LFG
CSB-BP312871LFG
CSB-MP312871LFG
CSB-EP312871LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 50S ribosomal protein L32 (rpmF) CSB-YP312872LFG
CSB-EP312872LFG
CSB-BP312872LFG
CSB-MP312872LFG
CSB-EP312872LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 50S ribosomal protein L33 2 (rpmG2) CSB-YP312873LFG
CSB-EP312873LFG
CSB-BP312873LFG
CSB-MP312873LFG
CSB-EP312873LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Arginine--tRNA ligase (argS), partial CSB-YP312874LFG
CSB-EP312874LFG
CSB-BP312874LFG
CSB-MP312874LFG
CSB-EP312874LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Chorismate synthase (aroC) CSB-YP312875LFG
CSB-EP312875LFG
CSB-BP312875LFG
CSB-MP312875LFG
CSB-EP312875LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Hydroxyethylthiazole kinase 2 (thiM2) CSB-YP312876LFG
CSB-EP312876LFG
CSB-BP312876LFG
CSB-MP312876LFG
CSB-EP312876LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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