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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae serotype 2 Tyrosine recombinase XerS (xerS) CSB-YP313040FNF
CSB-EP313040FNF
CSB-BP313040FNF
CSB-MP313040FNF
CSB-EP313040FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP313041FNF
CSB-EP313041FNF
CSB-BP313041FNF
CSB-MP313041FNF
CSB-EP313041FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 GTPase obg (obg) CSB-YP313042FNF
CSB-EP313042FNF
CSB-BP313042FNF
CSB-MP313042FNF
CSB-EP313042FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Peptidase T (pepT) CSB-YP313043FNF
CSB-EP313043FNF
CSB-BP313043FNF
CSB-MP313043FNF
CSB-EP313043FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Probable rRNA maturation factor (SPD_0855) CSB-YP313044FNF
CSB-EP313044FNF
CSB-BP313044FNF
CSB-MP313044FNF
CSB-EP313044FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Gamma-glutamyl phosphate reductase (proA) CSB-YP313045FNF
CSB-EP313045FNF
CSB-BP313045FNF
CSB-MP313045FNF
CSB-EP313045FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Pantothenate kinase (coaA) CSB-YP313046FNF
CSB-EP313046FNF
CSB-BP313046FNF
CSB-MP313046FNF
CSB-EP313046FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP313047FNF
CSB-EP313047FNF
CSB-BP313047FNF
CSB-MP313047FNF
CSB-EP313047FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 30S ribosomal protein S16 (rpsP) CSB-YP313048FNF
CSB-EP313048FNF
CSB-BP313048FNF
CSB-MP313048FNF
CSB-EP313048FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 50S ribosomal protein L1 (rplA) CSB-YP313050FNF
CSB-EP313050FNF
CSB-BP313050FNF
CSB-MP313050FNF
CSB-EP313050FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP313051FNF
CSB-EP313051FNF
CSB-BP313051FNF
CSB-MP313051FNF
CSB-EP313051FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 tRNA (guanine-N (7)-)-methyltransferase CSB-YP313052FNF
CSB-EP313052FNF
CSB-BP313052FNF
CSB-MP313052FNF
CSB-EP313052FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 DNA polymerase IV (dinB) CSB-YP313053FNF
CSB-EP313053FNF
CSB-BP313053FNF
CSB-MP313053FNF
CSB-EP313053FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP313054FNF
CSB-EP313054FNF
CSB-BP313054FNF
CSB-MP313054FNF
CSB-EP313054FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP313055FNF
CSB-EP313055FNF
CSB-BP313055FNF
CSB-MP313055FNF
CSB-EP313055FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Cell cycle protein GpsB (gpsB) CSB-YP313056FNF
CSB-EP313056FNF
CSB-BP313056FNF
CSB-MP313056FNF
CSB-EP313056FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 UPF0371 protein SPD_0310 (SPD_0310) CSB-YP313057FNF
CSB-EP313057FNF
CSB-BP313057FNF
CSB-MP313057FNF
CSB-EP313057FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 30S ribosomal protein S12 (rpsL) CSB-YP313058FNF
CSB-EP313058FNF
CSB-BP313058FNF
CSB-MP313058FNF
CSB-EP313058FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Translation initiation factor IF-1 (infA) CSB-YP313059FNF
CSB-EP313059FNF
CSB-BP313059FNF
CSB-MP313059FNF
CSB-EP313059FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 50S ribosomal protein L6 (rplF) CSB-YP313060FNF
CSB-EP313060FNF
CSB-BP313060FNF
CSB-MP313060FNF
CSB-EP313060FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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