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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Viola sororia Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP313244VDC
CSB-EP313244VDC
CSB-BP313244VDC
CSB-MP313244VDC
CSB-EP313244VDC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase MYLIP-B (mylipb) CSB-YP313245DIL
CSB-EP313245DIL
CSB-BP313245DIL
CSB-MP313245DIL
CSB-EP313245DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Putative deoxyribonuclease tatdn3-A (tatdn3-a) CSB-YP313246XBE
CSB-EP313246XBE
CSB-BP313246XBE
CSB-MP313246XBE
CSB-EP313246XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carsonella ruddii 30S ribosomal protein S5 (rpsE) CSB-YP313248CEG
CSB-EP313248CEG
CSB-BP313248CEG
CSB-MP313248CEG
CSB-EP313248CEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carsonella ruddii DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP313249CEG
CSB-EP313249CEG
CSB-BP313249CEG
CSB-MP313249CEG
CSB-EP313249CEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L34 (rpmH) CSB-YP313250XBB
CSB-EP313250XBB
CSB-BP313250XBB
CSB-MP313250XBB
CSB-EP313250XBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S21 (rpsU) CSB-YP313251XBB
CSB-EP313251XBB
CSB-BP313251XBB
CSB-MP313251XBB
CSB-EP313251XBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae A-factor-processing enzyme (STE23), partial CSB-YP313252SVG
CSB-EP313252SVG
CSB-BP313252SVG
CSB-MP313252SVG
CSB-EP313252SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arachis hypogaea Endochitinase 1B CSB-YP313253ANE
CSB-EP313253ANE
CSB-BP313253ANE
CSB-MP313253ANE
CSB-EP313253ANE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alnus incana Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP313254AHP
CSB-EP313254AHP
CSB-BP313254AHP
CSB-MP313254AHP
CSB-EP313254AHP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corylus cornuta Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP313255CQD
CSB-EP313255CQD
CSB-BP313255CQD
CSB-MP313255CQD
CSB-EP313255CQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Secale cereale 50S ribosomal protein L12-2, chloroplastic (RPL12-2) CSB-YP313256STH
CSB-EP313256STH
CSB-BP313256STH
CSB-MP313256STH
CSB-EP313256STH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae tRNA-dihydrouridine (20a/20b) synthase [NAD (P)+] CSB-YP313257SVG
CSB-EP313257SVG
CSB-BP313257SVG
CSB-MP313257SVG
CSB-EP313257SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Abrus precatorius Abrin-d, partial CSB-YP313258AAC
CSB-BP313258AAC
CSB-MP313258AAC
CSB-EP313258AAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Procyclic form-specific polypeptide B-alpha (PARPB) CSB-YP313259TQV
CSB-EP313259TQV
CSB-BP313259TQV
CSB-MP313259TQV
CSB-EP313259TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized mitochondrial outer membrane protein YPR098C (YPR098C), partial CSB-YP313260SVG1
CSB-EP313260SVG1
CSB-BP313260SVG1
CSB-MP313260SVG1
CSB-EP313260SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized membrane protein YPR109W (YPR109W), partial CSB-YP313262SVG
CSB-EP313262SVG
CSB-BP313262SVG
CSB-MP313262SVG
CSB-EP313262SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Required for respiratory growth protein 8, mitochondrial (RRG8) CSB-YP313263SVG
CSB-EP313263SVG
CSB-BP313263SVG
CSB-MP313263SVG
CSB-EP313263SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pesticidal crystal protein cry3Bb (cry3Bb), partial CSB-YP313264BRN
CSB-EP313264BRN
CSB-BP313264BRN
CSB-MP313264BRN
CSB-EP313264BRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 30S ribosomal protein S27ae (rps27ae) CSB-YP313265HTJ
CSB-EP313265HTJ
CSB-BP313265HTJ
CSB-MP313265HTJ
CSB-EP313265HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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