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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Ribosomal protein L11 methyltransferase (prmA) CSB-YP313082LES
CSB-EP313082LES
CSB-BP313082LES
CSB-MP313082LES
CSB-EP313082LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP313083LES
CSB-EP313083LES
CSB-BP313083LES
CSB-MP313083LES
CSB-EP313083LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP313084LES
CSB-EP313084LES
CSB-BP313084LES
CSB-MP313084LES
CSB-EP313084LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis ATP synthase gamma chain (atpG) CSB-YP313086LES
CSB-EP313086LES
CSB-BP313086LES
CSB-MP313086LES
CSB-EP313086LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Bifunctional protein FolD (folD) CSB-YP313087LES
CSB-EP313087LES
CSB-BP313087LES
CSB-MP313087LES
CSB-EP313087LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Enolase (eno) CSB-YP313088LES
CSB-EP313088LES
CSB-BP313088LES
CSB-MP313088LES
CSB-EP313088LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Ribosome maturation factor RimM (rimM) CSB-YP313089LES
CSB-EP313089LES
CSB-BP313089LES
CSB-MP313089LES
CSB-EP313089LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP313090LES
CSB-EP313090LES
CSB-BP313090LES
CSB-MP313090LES
CSB-EP313090LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP313091LES
CSB-EP313091LES
CSB-BP313091LES
CSB-MP313091LES
CSB-EP313091LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis CinA-like protein (LBJ_1205) CSB-YP313092LES
CSB-EP313092LES
CSB-BP313092LES
CSB-MP313092LES
CSB-EP313092LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 30S ribosomal protein S18 (rpsR) CSB-YP313093LES
CSB-EP313093LES
CSB-BP313093LES
CSB-MP313093LES
CSB-EP313093LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis GTPase Der (der) CSB-YP313094LES
CSB-EP313094LES
CSB-BP313094LES
CSB-MP313094LES
CSB-EP313094LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Tryptophan synthase alpha chain (trpA) CSB-YP313095LES
CSB-EP313095LES
CSB-BP313095LES
CSB-MP313095LES
CSB-EP313095LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP313096LES
CSB-EP313096LES
CSB-BP313096LES
CSB-MP313096LES
CSB-EP313096LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP313097LES
CSB-EP313097LES
CSB-BP313097LES
CSB-MP313097LES
CSB-EP313097LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP313098LES
CSB-EP313098LES
CSB-BP313098LES
CSB-MP313098LES
CSB-EP313098LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Type III pantothenate kinase (coaX) CSB-YP313099LES
CSB-EP313099LES
CSB-BP313099LES
CSB-MP313099LES
CSB-EP313099LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Chaperone protein DnaJ (dnaJ) CSB-YP313100LES
CSB-EP313100LES
CSB-BP313100LES
CSB-MP313100LES
CSB-EP313100LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 30S ribosomal protein S20 (rpsT) CSB-YP313101LES
CSB-EP313101LES
CSB-BP313101LES
CSB-MP313101LES
CSB-EP313101LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Putative membrane protein insertion efficiency factor (LBJ_0146) CSB-YP313102LES
CSB-EP313102LES
CSB-BP313102LES
CSB-MP313102LES
CSB-EP313102LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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