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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Protein translocase subunit SecY (secY), partial CSB-YP313775EGX
CSB-EP313775EGX
CSB-BP313775EGX
CSB-MP313775EGX
CSB-EP313775EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulatory protein uhpA (uhpA) CSB-YP313776SZB
CSB-EP313776SZB
CSB-BP313776SZB
CSB-MP313776SZB
CSB-EP313776SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli RNA polymerase sigma factor RpoH (rpoH) CSB-YP313777ENV
CSB-BP313777ENV
CSB-MP313777ENV
CSB-EP313777ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hexose phosphate transport protein (uhpT), partial CSB-YP313778SZB
CSB-EP313778SZB
CSB-BP313778SZB
CSB-MP313778SZB
CSB-EP313778SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein smp (smp) CSB-YP313779SZB
CSB-EP313779SZB
CSB-BP313779SZB
CSB-MP313779SZB
CSB-EP313779SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Signal recognition particle protein (ffh) CSB-YP313780EGX
CSB-EP313780EGX
CSB-BP313780EGX
CSB-MP313780EGX
CSB-EP313780EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Serine/threonine transporter sstT (sstT), partial CSB-YP313781ENV
CSB-EP313781ENV
CSB-BP313781ENV
CSB-MP313781ENV
CSB-EP313781ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Succinyl-CoA ligase [ADP-forming] subunit alpha (sucD) CSB-YP313782EOD
CSB-EP313782EOD
CSB-BP313782EOD
CSB-MP313782EOD
CSB-EP313782EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein tldD (tldD) CSB-YP313783ENV
CSB-EP313783ENV
CSB-BP313783ENV
CSB-MP313783ENV
CSB-EP313783ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide transport system permease protein sapB (sapB), partial CSB-YP313784ENV1
CSB-EP313784ENV1
CSB-BP313784ENV1
CSB-MP313784ENV1
CSB-EP313784ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphate transport system permease protein pstC (pstC), partial CSB-YP313785ENV1
CSB-EP313785ENV1
CSB-BP313785ENV1
CSB-MP313785ENV1
CSB-EP313785ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylose transport system permease protein xylH (xylH), partial CSB-YP313786EOD
CSB-EP313786EOD
CSB-BP313786EOD
CSB-MP313786EOD
CSB-EP313786EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative reactive intermediate deaminase TdcF (tdcF) CSB-YP313788ENV
CSB-EP313788ENV
CSB-BP313788ENV
CSB-MP313788ENV
CSB-EP313788ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein sirB1 (sirB1) CSB-YP313789ENV
CSB-EP313789ENV
CSB-BP313789ENV
CSB-MP313789ENV
CSB-EP313789ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthine permease XanP (xanP), partial CSB-YP313790EOD
CSB-EP313790EOD
CSB-BP313790EOD
CSB-MP313790EOD
CSB-EP313790EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kassina senegalensis Kassinatuerin-1 CSB-YP313791KAA
CSB-EP313791KAA
CSB-BP313791KAA
CSB-MP313791KAA
CSB-EP313791KAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP313792EOD
CSB-EP313792EOD
CSB-BP313792EOD
CSB-MP313792EOD
CSB-EP313792EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ubiquitin-NEDD8-like protein RUB1 (RUB1) CSB-YP313793OFG
CSB-EP313793OFG
CSB-BP313793OFG
CSB-MP313793OFG
CSB-EP313793OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP313794MSB
CSB-EP313794MSB
CSB-BP313794MSB
CSB-MP313794MSB
CSB-EP313794MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0345 protein YaiE (yaiE) CSB-YP313795EGX
CSB-EP313795EGX
CSB-BP313795EGX
CSB-MP313795EGX
CSB-EP313795EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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