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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Uncharacterized protein At4g15970 (At4g15970) CSB-YP313796DOA
CSB-EP313796DOA
CSB-BP313796DOA
CSB-MP313796DOA
CSB-EP313796DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Small heat shock protein ibpA (ibpA) CSB-YP313797ENV
CSB-EP313797ENV
CSB-BP313797ENV
CSB-MP313797ENV
CSB-EP313797ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small heat shock protein ibpA (ibpA) CSB-YP313798EGX
CSB-EP313798EGX
CSB-BP313798EGX
CSB-MP313798EGX
CSB-EP313798EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brevibacillus brevis Gramicidin S synthase 2 (grsB), partial CSB-YP313799BWK
CSB-EP313799BWK
CSB-BP313799BWK
CSB-MP313799BWK
CSB-EP313799BWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Multidrug/solvent efflux pump periplasmic linker protein mepA (mepA) CSB-YP313800FFZ
CSB-EP313800FFZ
CSB-BP313800FFZ
CSB-MP313800FFZ
CSB-EP313800FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Autophagy-related protein 8 (ATG8) CSB-YP313801CZD
CSB-EP313801CZD
CSB-BP313801CZD
CSB-MP313801CZD
CSB-EP313801CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia haemolytica Leukotoxin (lktA), partial CSB-YP313802ESE
CSB-EP313802ESE
CSB-BP313802ESE
CSB-MP313802ESE
CSB-EP313802ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica Leukotoxin translocation ATP-binding protein LktB (lktB), partial CSB-YP313803ESE1
CSB-EP313803ESE1
CSB-BP313803ESE1
CSB-MP313803ESE1
CSB-EP313803ESE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP313804SMT
CSB-EP313804SMT
CSB-BP313804SMT
CSB-MP313804SMT
CSB-EP313804SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes Arginine deiminase (arcA) CSB-YP313805SMR
CSB-EP313805SMR
CSB-BP313805SMR
CSB-MP313805SMR
CSB-EP313805SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP313806SMR
CSB-EP313806SMR
CSB-BP313806SMR
CSB-MP313806SMR
CSB-EP313806SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP313807SMT
CSB-EP313807SMT
CSB-BP313807SMT
CSB-MP313807SMT
CSB-EP313807SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trigger factor (tig) CSB-YP313808SMT
CSB-EP313808SMT
CSB-BP313808SMT
CSB-MP313808SMT
CSB-EP313808SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative sugar uptake protein SPy_2205/M5005_Spy1856 (SPy_2205, M5005_Spy1856), partial CSB-YP313809SMT
CSB-EP313809SMT
CSB-BP313809SMT
CSB-MP313809SMT
CSB-EP313809SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lantibiotic streptin (srtA) CSB-YP313810SMR
CSB-EP313810SMR
CSB-BP313810SMR
CSB-MP313810SMR
CSB-EP313810SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein rdoA (rdoA) CSB-YP313812EGX
CSB-EP313812EGX
CSB-BP313812EGX
CSB-MP313812EGX
CSB-EP313812EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable L-ascorbate peroxidase 5, chloroplastic (APX5) CSB-YP313813OFG
CSB-EP313813OFG
CSB-BP313813OFG
CSB-MP313813OFG
CSB-EP313813OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Accessory gene regulator protein B (agrB), partial CSB-YP313814FLL1
CSB-EP313814FLL1
CSB-BP313814FLL1
CSB-MP313814FLL1
CSB-EP313814FLL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Extracellular elastase (sepA) CSB-YP313815SLD
CSB-BP313815SLD
CSB-MP313815SLD
CSB-EP313815SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP313816ENV
CSB-EP313816ENV
CSB-BP313816ENV
CSB-MP313816ENV
CSB-EP313816ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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