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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Type VII secretion system accessory factor EsaA (esaA), partial CSB-YP314204FLG
CSB-BP314204FLG
CSB-MP314204FLG
CSB-EP314204FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small heat shock protein ibpB (ibpB) CSB-YP314205EGX
CSB-EP314205EGX
CSB-BP314205EGX
CSB-MP314205EGX
CSB-EP314205EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L31 (rpmE) CSB-YP314206EGX
CSB-EP314206EGX
CSB-BP314206EGX
CSB-MP314206EGX
CSB-EP314206EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antitoxin RelB (relB) CSB-YP314207SZB
CSB-EP314207SZB
CSB-BP314207SZB
CSB-MP314207SZB
CSB-EP314207SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Protein RecA (recA) CSB-YP314208FNH
CSB-EP314208FNH
CSB-BP314208FNH
CSB-MP314208FNH
CSB-EP314208FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L33 3 (rpmG3) CSB-YP314209FLB
CSB-EP314209FLB
CSB-BP314209FLB
CSB-MP314209FLB
CSB-EP314209FLB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine deiminase (arcA) CSB-YP314210SMT
CSB-EP314210SMT
CSB-BP314210SMT
CSB-MP314210SMT
CSB-EP314210SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP314211SMR
CSB-EP314211SMR
CSB-BP314211SMR
CSB-MP314211SMR
CSB-EP314211SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lantibiotic transport ATP-binding protein SrtF (srtF) CSB-YP314212SMR
CSB-EP314212SMR
CSB-BP314212SMR
CSB-MP314212SMR
CSB-EP314212SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP314213SMT
CSB-EP314213SMT
CSB-BP314213SMT
CSB-MP314213SMT
CSB-EP314213SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (folK) CSB-YP314214SMT
CSB-EP314214SMT
CSB-BP314214SMT
CSB-MP314214SMT
CSB-EP314214SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lantibiotic streptin (srtA) CSB-YP314215SMT
CSB-EP314215SMT
CSB-BP314215SMT
CSB-MP314215SMT
CSB-EP314215SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hyopneumoniae 30S ribosomal protein S15 (rpsO) CSB-YP314216MWK
CSB-EP314216MWK
CSB-BP314216MWK
CSB-MP314216MWK
CSB-EP314216MWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peroxiredoxin osmC (osmC) CSB-YP314217SZB
CSB-EP314217SZB
CSB-BP314217SZB
CSB-MP314217SZB
CSB-EP314217SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable indole-3-acetic acid-amido synthetase GH3.2 (GH3.2), partial CSB-YP314218OFG
CSB-EP314218OFG
CSB-BP314218OFG
CSB-MP314218OFG
CSB-EP314218OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 60 kDa chaperonin (groL), partial CSB-YP314219SLD
CSB-EP314219SLD
CSB-BP314219SLD
CSB-MP314219SLD
CSB-EP314219SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glutamyl endopeptidase (gseA) CSB-YP314220SLD
CSB-EP314220SLD
CSB-BP314220SLD
CSB-MP314220SLD
CSB-EP314220SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA gyrase subunit A (gyrA) CSB-YP314221FLL
CSB-EP314221FLL
CSB-BP314221FLL
CSB-MP314221FLL
CSB-EP314221FLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP314222SZB
CSB-EP314222SZB
CSB-BP314222SZB
CSB-MP314222SZB
CSB-EP314222SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillin-insensitive murein endopeptidase (mepA) CSB-YP314223SZB
CSB-EP314223SZB
CSB-BP314223SZB
CSB-MP314223SZB
CSB-EP314223SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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