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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant RNA polymerase sigma-32 factor (rpoH) CSB-YP314182EOD
CSB-EP314182EOD
CSB-BP314182EOD
CSB-MP314182EOD
CSB-EP314182EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soluble lytic murein transglycosylase (slt) CSB-YP314183EOD
CSB-EP314183EOD
CSB-BP314183EOD
CSB-MP314183EOD
CSB-EP314183EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Signal recognition particle protein (ffh) CSB-YP314184ENV
CSB-EP314184ENV
CSB-BP314184ENV
CSB-MP314184ENV
CSB-EP314184ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yieF (yieF) CSB-YP314185ENV
CSB-EP314185ENV
CSB-BP314185ENV
CSB-MP314185ENV
CSB-EP314185ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-xylose-proton symporter (xylE), partial CSB-YP314186ENV
CSB-EP314186ENV
CSB-BP314186ENV
CSB-MP314186ENV
CSB-EP314186ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-threonine dehydratase catabolic TdcB (tdcB) CSB-YP314187SZB
CSB-EP314187SZB
CSB-BP314187SZB
CSB-MP314187SZB
CSB-EP314187SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioredoxin-2 (trxC) CSB-YP314188EOD
CSB-EP314188EOD
CSB-BP314188EOD
CSB-MP314188EOD
CSB-EP314188EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide transport system permease protein sapB (sapB), partial CSB-YP314189EOD1
CSB-EP314189EOD1
CSB-BP314189EOD1
CSB-MP314189EOD1
CSB-EP314189EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate transport system permease protein pstC (pstC), partial CSB-YP314190EOD1
CSB-EP314190EOD1
CSB-BP314190EOD1
CSB-MP314190EOD1
CSB-EP314190EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose transport system permease protein rbsC (rbsC), partial CSB-YP314191EOD1
CSB-EP314191EOD1
CSB-BP314191EOD1
CSB-MP314191EOD1
CSB-EP314191EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylose transport system permease protein xylH (xylH), partial CSB-YP314192SZB
CSB-EP314192SZB
CSB-BP314192SZB
CSB-MP314192SZB
CSB-EP314192SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP314195EGX1
CSB-EP314195EGX1
CSB-BP314195EGX1
CSB-MP314195EGX1
CSB-EP314195EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator iscR (iscR) CSB-YP314196EOD
CSB-EP314196EOD
CSB-BP314196EOD
CSB-MP314196EOD
CSB-EP314196EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase E (rsmE) CSB-YP314197SZB
CSB-EP314197SZB
CSB-BP314197SZB
CSB-MP314197SZB
CSB-EP314197SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthine permease XanP (xanP), partial CSB-YP314198SZB
CSB-EP314198SZB
CSB-BP314198SZB
CSB-MP314198SZB
CSB-EP314198SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Morone chrysops x Morone saxatilis Piscidin-3 CSB-YP314199MIL
CSB-EP314199MIL
CSB-BP314199MIL
CSB-MP314199MIL
CSB-EP314199MIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L18 (rplR) CSB-YP314200ENV
CSB-EP314200ENV
CSB-BP314200ENV
CSB-MP314200ENV
CSB-EP314200ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ubiquitin-NEDD8-like protein RUB2 (RUB2) CSB-YP314201OFG
CSB-EP314201OFG
CSB-BP314201OFG
CSB-MP314201OFG
CSB-EP314201OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0345 protein YaiE (yaiE) CSB-YP314202ENV
CSB-EP314202ENV
CSB-BP314202ENV
CSB-MP314202ENV
CSB-EP314202ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis C virus genotype 1a F protein CSB-YP314203HFD
CSB-EP314203HFD
CSB-BP314203HFD
CSB-MP314203HFD
CSB-EP314203HFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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