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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Androctonus crassicauda Toxin Acra II-1 CSB-YP314816AKA
CSB-EP314816AKA
CSB-BP314816AKA
CSB-MP314816AKA
CSB-EP314816AKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Thiol:disulfide interchange protein DsbA (dsbA) CSB-YP314817EZX
CSB-EP314817EZX
CSB-BP314817EZX
CSB-MP314817EZX
CSB-EP314817EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Ribulose bisphosphate carboxylase large chain, chromosomal (cbbL1) CSB-YP314818CUS
CSB-EP314818CUS
CSB-BP314818CUS
CSB-MP314818CUS
CSB-EP314818CUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine decarboxylase proenzyme (hdc) CSB-YP314819CMB
CSB-EP314819CMB
CSB-BP314819CMB
CSB-MP314819CMB
CSB-EP314819CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens Perfringolysin O (pfo) CSB-YP314820CMB
CSB-BP314820CMB
CSB-MP314820CMB
CSB-EP314820CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative ribonuclease H protein At1g65750 (At1g65750), partial CSB-YP314821DOA
CSB-EP314821DOA
CSB-BP314821DOA
CSB-MP314821DOA
CSB-EP314821DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein At1g06630 (At1g06630) CSB-YP314822DOA
CSB-EP314822DOA
CSB-BP314822DOA
CSB-MP314822DOA
CSB-EP314822DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Uncharacterized membrane protein SPD_2301 (SPD_2301), partial CSB-YP314823FNF
CSB-EP314823FNF
CSB-BP314823FNF
CSB-MP314823FNF
CSB-EP314823FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L27-A (RPL27A) CSB-YP314824SVG
CSB-EP314824SVG
CSB-BP314824SVG
CSB-MP314824SVG
CSB-EP314824SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-PR1 Gag-Pol polyprotein (TY1B-PR1), partial CSB-YP314825SVG
CSB-EP314825SVG
CSB-BP314825SVG
CSB-MP314825SVG
CSB-EP314825SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty2-LR2 Gag polyprotein (TY2A-LR2) CSB-YP314826SVG
CSB-EP314826SVG
CSB-BP314826SVG
CSB-MP314826SVG
CSB-EP314826SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxosceles gaucho Sphingomyelin phosphodiesterase D LgSicTox-alphaI-1 CSB-YP314827LJE
CSB-EP314827LJE
CSB-BP314827LJE
CSB-MP314827LJE
CSB-EP314827LJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxosceles gaucho Sphingomyelin phosphodiesterase D LgSicTox-LOXN6 CSB-YP314828LJE
CSB-EP314828LJE
CSB-BP314828LJE
CSB-MP314828LJE
CSB-EP314828LJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxosceles laeta Sphingomyelin phosphodiesterase D LlSicTox-alphaIII-1 CSB-YP314829LRB
CSB-EP314829LRB
CSB-BP314829LRB
CSB-MP314829LRB
CSB-EP314829LRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP314830IGE
CSB-EP314830IGE
CSB-BP314830IGE
CSB-MP314830IGE
CSB-EP314830IGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein yscB (yscB) CSB-YP314831YAQ
CSB-EP314831YAQ
CSB-BP314831YAQ
CSB-MP314831YAQ
CSB-EP314831YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein yscY (yscY) CSB-YP314832YAQ
CSB-EP314832YAQ
CSB-BP314832YAQ
CSB-MP314832YAQ
CSB-EP314832YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blarina brevicauda Soricidin CSB-YP314833BTI
CSB-EP314833BTI
CSB-BP314833BTI
CSB-MP314833BTI
CSB-EP314833BTI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine hemagglutinating encephalomyelitis virus Envelope small membrane protein (E), partial CSB-YP314834PPY1
CSB-EP314834PPY1
CSB-BP314834PPY1
CSB-MP314834PPY1
CSB-EP314834PPY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Truncated non-structural protein of 4.9 kDa (4a) CSB-YP314835BJM
CSB-EP314835BJM
CSB-BP314835BJM
CSB-MP314835BJM
CSB-EP314835BJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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