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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella abortus biovar 1 Type IV secretion system protein virB6 (virB6), partial CSB-YP314347BWS1
CSB-EP314347BWS1
CSB-BP314347BWS1
CSB-MP314347BWS1
CSB-EP314347BWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus 133/2005 Non-structural protein 3b (3b) CSB-YP314348BFA
CSB-EP314348BFA
CSB-BP314348BFA
CSB-MP314348BFA
CSB-EP314348BFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Type IV secretion system protein virB9 (virB9) CSB-YP314349BWS
CSB-EP314349BWS
CSB-BP314349BWS
CSB-MP314349BWS
CSB-EP314349BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Type IV secretion system putative lipoprotein virB7 (virB7) CSB-YP314350BMO
CSB-EP314350BMO
CSB-BP314350BMO
CSB-MP314350BMO
CSB-EP314350BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L15 (rplO) CSB-YP314351DWY
CSB-EP314351DWY
CSB-BP314351DWY
CSB-MP314351DWY
CSB-EP314351DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Catalase isozyme A CSB-YP314352OFF
CSB-EP314352OFF
CSB-BP314352OFF
CSB-MP314352OFF
CSB-EP314352OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus fasciatus Neurotoxin 3FTx-RK CSB-YP314353BXL
CSB-EP314353BXL
CSB-BP314353BXL
CSB-MP314353BXL
CSB-EP314353BXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis DNA protection during starvation protein (dps) CSB-YP314354MVW
CSB-BP314354MVW
CSB-MP314354MVW
CSB-EP314354MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L34 (rpmH) CSB-YP314355MVW
CSB-EP314355MVW
CSB-BP314355MVW
CSB-MP314355MVW
CSB-EP314355MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0232 protein in recF-gyrB intergenic region CSB-YP314356MVW
CSB-EP314356MVW
CSB-BP314356MVW
CSB-MP314356MVW
CSB-EP314356MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mokola virus Phosphoprotein (P) CSB-YP314357MHF
CSB-EP314357MHF
CSB-BP314357MHF
CSB-MP314357MHF
CSB-EP314357MHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype D subtype ayw External core antigen (C) CSB-YP314358HEM
CSB-EP314358HEM
CSB-BP314358HEM
CSB-MP314358HEM
CSB-EP314358HEM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bitis gabonica Thrombin-like enzyme gabonase, partial CSB-YP314359BGW
CSB-BP314359BGW
CSB-MP314359BGW
CSB-EP314359BGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycolicibacterium smegmatis Catalase-peroxidase (katG), partial CSB-YP314360MVW
CSB-EP314360MVW
CSB-BP314360MVW
CSB-MP314360MVW
CSB-EP314360MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Granule-bound starch synthase 1, chloroplastic/amyloplastic (WAXY), partial CSB-YP314361OCO
CSB-EP314361OCO
CSB-BP314361OCO
CSB-MP314361OCO
CSB-EP314361OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viola arvensis Pro-tricyclons CSB-YP314362VCY
CSB-EP314362VCY
CSB-BP314362VCY
CSB-MP314362VCY
CSB-EP314362VCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus 279/2005 Protein 9b (9b) CSB-YP314363BFB
CSB-EP314363BFB
CSB-BP314363BFB
CSB-MP314363BFB
CSB-EP314363BFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica MADS-box transcription factor 7 (MADS7) CSB-YP314364OFF
CSB-EP314364OFF
CSB-BP314364OFF
CSB-MP314364OFF
CSB-EP314364OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius shedaoensis Thrombin-like enzyme gloshedobin CSB-YP314365GCO
CSB-EP314365GCO
CSB-BP314365GCO
CSB-MP314365GCO
CSB-EP314365GCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 1-Cys peroxiredoxin A (OsI_27030) CSB-YP314367OFF
CSB-EP314367OFF
CSB-BP314367OFF
CSB-MP314367OFF
CSB-EP314367OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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