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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Probable L-ascorbate peroxidase 6, chloroplastic (APX6) CSB-YP314756OFG
CSB-EP314756OFG
CSB-BP314756OFG
CSB-MP314756OFG
CSB-EP314756OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Proline/betaine transporter (proP), partial CSB-YP314757ENV
CSB-EP314757ENV
CSB-BP314757ENV
CSB-MP314757ENV
CSB-EP314757ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable indole-3-acetic acid-amido synthetase GH3.11 (GH3.11), partial CSB-YP314758OFG
CSB-EP314758OFG
CSB-BP314758OFG
CSB-MP314758OFG
CSB-EP314758OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Extracellular cysteine protease (ecpA) CSB-YP314759FLL
CSB-EP314759FLL
CSB-BP314759FLL
CSB-MP314759FLL
CSB-EP314759FLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Ras modification protein ERF4 (ERF4) CSB-YP314760YAP
CSB-EP314760YAP
CSB-BP314760YAP
CSB-MP314760YAP
CSB-EP314760YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella choleraesuis Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP314761SBF
CSB-EP314761SBF
CSB-BP314761SBF
CSB-MP314761SBF
CSB-EP314761SBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Small-conductance mechanosensitive channel (mscS), partial CSB-YP314762ENV1
CSB-EP314762ENV1
CSB-BP314762ENV1
CSB-MP314762ENV1
CSB-EP314762ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymantria dispar Chorion class A protein Ld2/Ld41 CSB-YP314763LKU
CSB-EP314763LKU
CSB-BP314763LKU
CSB-MP314763LKU
CSB-EP314763LKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drymarchon corais erebennus Hemoglobin subunit beta-2 CSB-YP314764DHD
CSB-EP314764DHD
CSB-BP314764DHD
CSB-MP314764DHD
CSB-EP314764DHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S21-A (RPS21A) CSB-YP314765SVG
CSB-EP314765SVG
CSB-BP314765SVG
CSB-MP314765SVG
CSB-EP314765SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina barkeri Monomethylamine methyltransferase mtmB3 (mtmB3) CSB-YP314766MSM
CSB-EP314766MSM
CSB-BP314766MSM
CSB-MP314766MSM
CSB-EP314766MSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable auxin efflux carrier component 1b (PIN1B), partial CSB-YP314767OFG
CSB-EP314767OFG
CSB-BP314767OFG
CSB-MP314767OFG
CSB-EP314767OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alternaria alternata Probable NADP-dependent mannitol dehydrogenase CSB-YP314768AZV
CSB-BP314768AZV
CSB-MP314768AZV
CSB-EP314768AZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Sensor histidine kinase regB (regB), partial CSB-YP314769RLE1
CSB-EP314769RLE1
CSB-BP314769RLE1
CSB-MP314769RLE1
CSB-EP314769RLE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Chaperonin GroEL (groEL) CSB-YP314770DSB
CSB-BP314770DSB
CSB-MP314770DSB
CSB-EP314770DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Outer membrane lipoprotein omp19 (omp19) CSB-YP314771BWS
CSB-EP314771BWS
CSB-BP314771BWS
CSB-MP314771BWS
CSB-EP314771BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Modification methylase BabI (ccrM) CSB-YP314772BWS
CSB-EP314772BWS
CSB-BP314772BWS
CSB-MP314772BWS
CSB-EP314772BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Protein-export protein SecB (secB) CSB-YP314773BMN
CSB-EP314773BMN
CSB-BP314773BMN
CSB-MP314773BMN
CSB-EP314773BMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Nuclear export protein (NS) CSB-YP314774IKF
CSB-EP314774IKF
CSB-BP314774IKF
CSB-MP314774IKF
CSB-EP314774IKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Non-structural protein 1 (NS) CSB-YP314775IKP
CSB-EP314775IKP
CSB-BP314775IKP
CSB-MP314775IKP
CSB-EP314775IKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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