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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Encephalitozoon intestinalis Polar tube protein 2 (PTP2) CSB-YP315969EIX
CSB-EP315969EIX
CSB-BP315969EIX
CSB-MP315969EIX
CSB-EP315969EIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus DnaK suppressor protein homolog (dksA) CSB-YP315970DQO
CSB-EP315970DQO
CSB-BP315970DQO
CSB-MP315970DQO
CSB-EP315970DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus DNA mismatch repair protein mutL (mutL), partial CSB-YP315971DQO
CSB-EP315971DQO
CSB-BP315971DQO
CSB-MP315971DQO
CSB-EP315971DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 30S ribosomal protein S20 (rpsT) CSB-YP315972DQO
CSB-EP315972DQO
CSB-BP315972DQO
CSB-MP315972DQO
CSB-EP315972DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus RNA polymerase sigma-32 factor (rpoH) CSB-YP315973DQO
CSB-EP315973DQO
CSB-BP315973DQO
CSB-MP315973DQO
CSB-EP315973DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative defensin-like protein 53 (At2g34135) CSB-YP315974DOA
CSB-EP315974DOA
CSB-BP315974DOA
CSB-MP315974DOA
CSB-EP315974DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetrahymena thermophila Tubulin glycylase 3F (TTLL3F), partial CSB-YP315975TKA
CSB-EP315975TKA
CSB-BP315975TKA
CSB-MP315975TKA
CSB-EP315975TKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachesana tarabaevi M-zodatoxin-Lt8o (cit 1-14) CSB-YP315976LAF
CSB-EP315976LAF
CSB-BP315976LAF
CSB-MP315976LAF
CSB-EP315976LAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calloselasma rhodostoma Zinc metalloproteinase kistomin CSB-YP315977CBG
CSB-EP315977CBG
CSB-BP315977CBG
CSB-MP315977CBG
CSB-EP315977CBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g38062 (At4g38062), partial CSB-YP315978DOA
CSB-EP315978DOA
CSB-BP315978DOA
CSB-MP315978DOA
CSB-EP315978DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At1g10895 (At1g10895) CSB-YP315979DOA
CSB-EP315979DOA
CSB-BP315979DOA
CSB-MP315979DOA
CSB-EP315979DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus GTPase obg (obg) CSB-YP315980DQO
CSB-EP315980DQO
CSB-BP315980DQO
CSB-MP315980DQO
CSB-EP315980DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP315981FMW
CSB-EP315981FMW
CSB-BP315981FMW
CSB-MP315981FMW
CSB-EP315981FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (NDUFB8), partial CSB-YP315983EXP1
CSB-EP315983EXP1
CSB-BP315983EXP1
CSB-MP315983EXP1
CSB-EP315983EXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP315984FMW
CSB-EP315984FMW
CSB-BP315984FMW
CSB-MP315984FMW
CSB-EP315984FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lasiodora parahybana U1-theraphotoxin-Lp1a CSB-YP315985LCN
CSB-EP315985LCN
CSB-BP315985LCN
CSB-MP315985LCN
CSB-EP315985LCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum PAB-dependent poly (A)-specific ribonuclease subunit 3, partial CSB-YP315986DKK
CSB-EP315986DKK
CSB-BP315986DKK
CSB-MP315986DKK
CSB-EP315986DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP315987DSB
CSB-EP315987DSB
CSB-BP315987DSB
CSB-MP315987DSB
CSB-EP315987DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis 50S ribosomal protein L17 (rplQ) CSB-YP315988DSB
CSB-EP315988DSB
CSB-BP315988DSB
CSB-MP315988DSB
CSB-EP315988DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Aquaporin-like protein 2 (AQY2), partial CSB-YP315989SVG
CSB-EP315989SVG
CSB-BP315989SVG
CSB-MP315989SVG
CSB-EP315989SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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