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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neurospora crassa Patatin-like phospholipase domain-containing protein NCU11180 (NCU00381, NCU11180), partial CSB-YP315042NHA
CSB-EP315042NHA
CSB-BP315042NHA
CSB-MP315042NHA
CSB-EP315042NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Protein I (I) CSB-YP315043MKA
CSB-EP315043MKA
CSB-BP315043MKA
CSB-MP315043MKA
CSB-EP315043MKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Non-structural protein 4 (4), partial CSB-YP315044MJY1
CSB-EP315044MJY1
CSB-BP315044MJY1
CSB-MP315044MJY1
CSB-EP315044MJY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human coronavirus HKU1 Protein I (N) CSB-YP315045HIV
CSB-EP315045HIV
CSB-BP315045HIV
CSB-MP315045HIV
CSB-EP315045HIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP315046MVZ
CSB-EP315046MVZ
CSB-BP315046MVZ
CSB-MP315046MVZ
CSB-EP315046MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycolic acid methyltransferase MmaA1 (mmaA1) CSB-YP315047MVZ
CSB-EP315047MVZ
CSB-BP315047MVZ
CSB-MP315047MVZ
CSB-EP315047MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA gyrase subunit B (gyrB), partial CSB-YP315048MVZ
CSB-EP315048MVZ
CSB-BP315048MVZ
CSB-MP315048MVZ
CSB-EP315048MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 1-Cys peroxiredoxin B (Os07g0638400, LOC_Os07g44440) CSB-YP315049OFG
CSB-EP315049OFG
CSB-BP315049OFG
CSB-MP315049OFG
CSB-EP315049OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peroxiredoxin Q, chloroplastic (Os06g0196300, LOC_Os06g09610) CSB-YP315050OFG
CSB-EP315050OFG
CSB-BP315050OFG
CSB-MP315050OFG
CSB-EP315050OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaetomium globosum Protein FMP52, mitochondrial (FMP52) CSB-YP315051CHI
CSB-EP315051CHI
CSB-BP315051CHI
CSB-MP315051CHI
CSB-EP315051CHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable serine/threonine-protein kinase At1g18390 (At1g18390), partial CSB-YP315052DOA
CSB-EP315052DOA
CSB-BP315052DOA
CSB-MP315052DOA
CSB-EP315052DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabuthus granulatus Toxin PgKL2 CSB-YP315053ERL
CSB-EP315053ERL
CSB-BP315053ERL
CSB-MP315053ERL
CSB-EP315053ERL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liocheles australasiae Venom peptide La1 CSB-YP315054LGK
CSB-EP315054LGK
CSB-BP315054LGK
CSB-MP315054LGK
CSB-EP315054LGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura sp. Toxin Hk8 CSB-YP315055ALN
CSB-EP315055ALN
CSB-BP315055ALN
CSB-MP315055ALN
CSB-EP315055ALN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura xanthogrammica Toxin PCR4 CSB-YP315056ALO
CSB-EP315056ALO
CSB-BP315056ALO
CSB-MP315056ALO
CSB-EP315056ALO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura xanthogrammica Toxin PCR6 CSB-YP315057ALO
CSB-EP315057ALO
CSB-BP315057ALO
CSB-MP315057ALO
CSB-EP315057ALO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halcurias carlgreni Halcurin CSB-YP315058HAR
CSB-EP315058HAR
CSB-BP315058HAR
CSB-MP315058HAR
CSB-EP315058HAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Gibberellin 20 oxidase 2 (20ox2) CSB-YP315059OFF
CSB-EP315059OFF
CSB-BP315059OFF
CSB-MP315059OFF
CSB-EP315059OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Beta-insect depressant toxin Lqh-dprIT3a CSB-YP315060LDS
CSB-EP315060LDS
CSB-BP315060LDS
CSB-MP315060LDS
CSB-EP315060LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Beta-insect depressant toxin Lqh-dprIT3d CSB-YP315061LDS
CSB-EP315061LDS
CSB-BP315061LDS
CSB-MP315061LDS
CSB-EP315061LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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