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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Protein perB (perB) CSB-YP315443ENZ
CSB-EP315443ENZ
CSB-BP315443ENZ
CSB-MP315443ENZ
CSB-EP315443ENZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Probable methyltransferase EP424R (Ken-067) CSB-YP315444AEB
CSB-EP315444AEB
CSB-BP315444AEB
CSB-MP315444AEB
CSB-EP315444AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative DNA-directed RNA polymerase subunit 10 homolog (Pret-107) CSB-YP315445AYG
CSB-EP315445AYG
CSB-BP315445AYG
CSB-MP315445AYG
CSB-EP315445AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Repair DNA polymerase X (Ken-109) CSB-YP315446AEB
CSB-EP315446AEB
CSB-BP315446AEB
CSB-MP315446AEB
CSB-EP315446AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Probable mRNA-capping enzyme (Pret-113), partial CSB-YP315447AYG
CSB-EP315447AYG
CSB-BP315447AYG
CSB-MP315447AYG
CSB-EP315447AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative ATP-dependent RNA helicase QP509L (Pret-135), partial CSB-YP315448AYG
CSB-EP315448AYG
CSB-BP315448AYG
CSB-MP315448AYG
CSB-EP315448AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative DNA-directed RNA polymerase subunit 5 homolog (Pret-120) CSB-YP315449AYG
CSB-EP315449AYG
CSB-BP315449AYG
CSB-MP315449AYG
CSB-EP315449AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative poly (A) polymerase catalytic subunit CSB-YP315451AYE
CSB-EP315451AYE
CSB-BP315451AYE
CSB-MP315451AYE
CSB-EP315451AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus DNA-directed RNA polymerase subunit 6 homolog (Ken-081) CSB-YP315452AEB
CSB-EP315452AEB
CSB-BP315452AEB
CSB-MP315452AEB
CSB-EP315452AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 100-1R (War-018) CSB-YP315453AEI
CSB-EP315453AEI
CSB-BP315453AEI
CSB-MP315453AEI
CSB-EP315453AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 100-2L (Ken-162) CSB-YP315454AEB
CSB-EP315454AEB
CSB-BP315454AEB
CSB-MP315454AEB
CSB-EP315454AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-1L (Ken-007), partial CSB-YP315455AEB1
CSB-EP315455AEB1
CSB-BP315455AEB1
CSB-MP315455AEB1
CSB-EP315455AEB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-4L (War-009) CSB-YP315456AEI
CSB-EP315456AEI
CSB-BP315456AEI
CSB-MP315456AEI
CSB-EP315456AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-6L (Ken-013) CSB-YP315457AEB
CSB-EP315457AEB
CSB-BP315457AEB
CSB-MP315457AEB
CSB-EP315457AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-9L (War-019), partial CSB-YP315458AEI1
CSB-EP315458AEI1
CSB-BP315458AEI1
CSB-MP315458AEI1
CSB-EP315458AEI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 360-3L (Mal-016), partial CSB-YP315459AYE1
CSB-EP315459AYE1
CSB-BP315459AYE1
CSB-MP315459AYE1
CSB-EP315459AYE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 300-2R (War-026) CSB-YP315460AEI
CSB-EP315460AEI
CSB-BP315460AEI
CSB-MP315460AEI
CSB-EP315460AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 360-3L (Mal-004) CSB-YP315461AYE
CSB-EP315461AYE
CSB-BP315461AYE
CSB-MP315461AYE
CSB-EP315461AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 360-5L (Pret-025) CSB-YP315462AYG
CSB-EP315462AYG
CSB-BP315462AYG
CSB-MP315462AYG
CSB-EP315462AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 360-8L (Mal-027) CSB-YP315463AYE
CSB-EP315463AYE
CSB-BP315463AYE
CSB-MP315463AYE
CSB-EP315463AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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